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中华损伤与修复杂志(电子版) ›› 2020, Vol. 15 ›› Issue (06) : 423 -427. doi: 10.3877/cma.j.issn.1673-9450.2020.06.001

所属专题: 文献

专家述评

困惑、挑战与希望
贾赤宇1,(), 毛舒婷1   
  1. 1. 361102 厦门大学附属翔安医院烧伤整形与创面修复科
  • 收稿日期:2020-10-26 出版日期:2020-12-01
  • 通信作者: 贾赤宇
  • 基金资助:
    福建省自然科学基金(2019J01011)

Confusion, challenge and hope on diagnosis of tuberculous wound

Chiyu Jia1,(), Shuting Mao1   

  1. 1. Department of Burns and Plastic & Wound Repair Surgery, Xiang′an Hospital of Xiamen University, Xiamen 361102, China
  • Received:2020-10-26 Published:2020-12-01
  • Corresponding author: Chiyu Jia
  • About author:
    Corresponding author: Jia Chiyu, Email:
引用本文:

贾赤宇, 毛舒婷. 困惑、挑战与希望[J]. 中华损伤与修复杂志(电子版), 2020, 15(06): 423-427.

Chiyu Jia, Shuting Mao. Confusion, challenge and hope on diagnosis of tuberculous wound[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2020, 15(06): 423-427.

结核性创面的临床诊断依然是困扰医师实施精确治疗的难题。结核分枝杆菌分离培养、抗酸染色、病理组织学检查、结核菌素试验、色谱法、血清免疫学法和分子生物学诊断等传统的诊断方法虽然均在应用,但普遍存在操作过程复杂、周期较长、检测阳性率较低等缺陷。寻求一种快速、方便、简洁及检测阳性率高的方法是结核性创面临床诊断面临的挑战。新兴分子生物学技术——实时荧光定量聚合酶链反应(PCR)探针熔解曲线法在结核性创面石蜡组织中的诊断和耐药分析中展示出独特的优越性:用时短、操作简便,能够同时鉴定多种结核分枝杆菌及非结核分枝杆菌,特异度、敏感度高,为解决结核性创面临床诊断难题带来了新希望。

The clinical diagnosis of tuberculous wound is still a difficult problem for doctors to implement accurate treatment. Although some traditional diagnostic methods like mycobacterium tuberculosis isolation and culture, acid-fast staining, histopheological examination, tuberculin test, chromatography, serum immunology and molecular biology diagnosis are all in use, there are generally defects such as complex operation process, long cycle and low positive rate. The challenge in clinical diagnosis of tuberculous wound is to find a rapid, convenient, concise and highly positive method. New molecular biology technology real-time fluorescence quantitative polymerase chain reaction (PCR) probe fusion curve method has shown its unique advantages in the diagnosis and drug resistance analysis of the paraffin-embedded tissue of tuberculous wound. It is short in time, easy to operate, and can identify multiple mycobacterium tuberculosis and non-mycobacterium tuberculosis at the same time, with high specificity and sensitivity. It brings new hope for the difficult problem of clinical diagnosis of tuberculous wound.

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