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中华损伤与修复杂志(电子版) ›› 2020, Vol. 15 ›› Issue (06) : 423 -427. doi: 10.3877/cma.j.issn.1673-9450.2020.06.001

所属专题: 文献

专家述评

困惑、挑战与希望
贾赤宇1,(), 毛舒婷1   
  1. 1. 361102 厦门大学附属翔安医院烧伤整形与创面修复科
  • 收稿日期:2020-10-26 出版日期:2020-12-01
  • 通信作者: 贾赤宇
  • 基金资助:
    福建省自然科学基金(2019J01011)

Confusion, challenge and hope on diagnosis of tuberculous wound

Chiyu Jia1,(), Shuting Mao1   

  1. 1. Department of Burns and Plastic & Wound Repair Surgery, Xiang′an Hospital of Xiamen University, Xiamen 361102, China
  • Received:2020-10-26 Published:2020-12-01
  • Corresponding author: Chiyu Jia
  • About author:
    Corresponding author: Jia Chiyu, Email:
引用本文:

贾赤宇, 毛舒婷. 困惑、挑战与希望[J/OL]. 中华损伤与修复杂志(电子版), 2020, 15(06): 423-427.

Chiyu Jia, Shuting Mao. Confusion, challenge and hope on diagnosis of tuberculous wound[J/OL]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2020, 15(06): 423-427.

结核性创面的临床诊断依然是困扰医师实施精确治疗的难题。结核分枝杆菌分离培养、抗酸染色、病理组织学检查、结核菌素试验、色谱法、血清免疫学法和分子生物学诊断等传统的诊断方法虽然均在应用,但普遍存在操作过程复杂、周期较长、检测阳性率较低等缺陷。寻求一种快速、方便、简洁及检测阳性率高的方法是结核性创面临床诊断面临的挑战。新兴分子生物学技术——实时荧光定量聚合酶链反应(PCR)探针熔解曲线法在结核性创面石蜡组织中的诊断和耐药分析中展示出独特的优越性:用时短、操作简便,能够同时鉴定多种结核分枝杆菌及非结核分枝杆菌,特异度、敏感度高,为解决结核性创面临床诊断难题带来了新希望。

The clinical diagnosis of tuberculous wound is still a difficult problem for doctors to implement accurate treatment. Although some traditional diagnostic methods like mycobacterium tuberculosis isolation and culture, acid-fast staining, histopheological examination, tuberculin test, chromatography, serum immunology and molecular biology diagnosis are all in use, there are generally defects such as complex operation process, long cycle and low positive rate. The challenge in clinical diagnosis of tuberculous wound is to find a rapid, convenient, concise and highly positive method. New molecular biology technology real-time fluorescence quantitative polymerase chain reaction (PCR) probe fusion curve method has shown its unique advantages in the diagnosis and drug resistance analysis of the paraffin-embedded tissue of tuberculous wound. It is short in time, easy to operate, and can identify multiple mycobacterium tuberculosis and non-mycobacterium tuberculosis at the same time, with high specificity and sensitivity. It brings new hope for the difficult problem of clinical diagnosis of tuberculous wound.

[1]
罗一婷,翁榕星,周芳,等. 2019 WHO全球结核报告:全球与中国关键数据分析[J/CD]. 新发传染病电子杂志,2020, 5(1): 47-50.
[2]
贾赤宇. 结核性创面——一个被忽视且值得重视的临床问题[J/CD]. 中华损伤与修复杂志(电子版), 2014, 9(4): 355-359.
[3]
Davies PD, Pai M. The diagnosis and misdiagnosis of tuberculosis[J]. Int J Tuberc Lung Dis, 2008, 12(11): 1226-1234.
[4]
Almaguer-Chávez J, Ocampo-Candiani J, Rendón A. Current panorama in the diagnosis of cutaneous tuberculosis[J]. Actas Dermosifiliogr, 2009, 100(7): 562-570.
[5]
Caminero Luna JA, Casal Román M, Casal Román M, et al. Tuberculosis diagnosis[J]. Arch Bronconeumol, 1996, 32(2): 85-99.
[6]
Desai AM, Hsu S. Medical pearl: Interpretation of tuberculin skin tests in patients who have received the BCG vaccine[J]. J Am Acad Dermatol, 2005, 53(5): 868-869.
[7]
Mazurek GH, Jereb J, Lobue P, et al. Guidelines for using the QuantiFERON-TB Gold test for detecting Mycobacterium tuberculosis infection, United States[J]. MMWR Recomm Rep, 2005, 54(RR-15): 49-55.
[8]
Hill PC, Brookes RH, Fox A, et al. Longitudinal assessment of an ELISPOT test for Mycobacterium tuberculosis infection[J]. PLoS Med, 2007, 4(6): e192.
[9]
van Pinxteren LA, Ravn P, Agger EM, et al. Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10[J]. Clin Diagn Lab Immunol, 2000, 7(2): 155-160.
[10]
Ferrara G, Losi M, Losi M, et al. Use in routine clinical practice of two commercial blood tests for diagnosis of infection with Mycobacterium tuberculosis: a prospective study[J]. Lancet, 2006, 367(9519): 1328-1334.
[11]
Posteraro B, Sanguinetti M, Garcovich A, et al. Polymerase chain reaction-reverse cross-blot hybridization assay in the diagnosis of sporotrichoid Mycobacterium marinum infection[J]. Br J Dermatol, 1998, 139(5): 872-876.
[12]
白洁,张全华,颉玉胜,等. 皮肤结核诊断的新进展[J]. 医学综述,2015, 21(6): 1038-1040.
[13]
Afsar I, Afsar FS. Evaluation of laboratory diagnosis for cutaneous tuberculosis[J]. Indian J Pathol Microbiol, 2016, 59(3): 274-278.
[14]
Agarwal P, Singh EN, Agarwal US, et al. The role of DNA polymerase chain reaction, culture and histopathology in the diagnosis of cutaneous tuberculosis[J]. Int J Dermatol, 2017, 56(11): 1119-1124.
[15]
张浩,刘永芳,刘成才,等. 皮肤结核28例回顾分析[J]. 中国麻风皮肤病杂志,2005, 21(2): 108-109.
[16]
Huang Q, Liu Z, Liao Y, et al. Multiplex fluorescence melting curve analysis for mutation detection with dual-labeled, self-quenched probes[J]. PLoS One, 2011, 6(4): e19206.
[17]
Liao Y, Wang X, Sha C, et al. Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection[J]. Nucleic Acids Res, 2013, 41(7): e76.
[18]
Cloud JL, Neal H, Rosenberry R, et al. Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries[J]. J Clin Microbiol, 2002, 40(2): 400-406.
[19]
Xu Y, Liang B, Du C, et al. Rapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis[J]. J Clin Microbiol, 2019, 57(1): e01096-e010918.
[20]
Tang Z, Xu Y, Song N, et al. A comparison of the MeltPro? HPV Test with the Cobas® HPV Test for detecting and genotyping 14 high-risk human papillomavirus types[J]. Arch Virol, 2018, 163(3): 725-730.
[21]
Huang Q, Wang X, Tang N, et al. Rapid detection of non-deletional mutations causing α-thalassemia by multicolor melting curve analysis[J]. Clin Chem Lab Med, 2016, 54(3): 397-402.
[22]
Mou Y, Athar MA, Wu Y, et al. Detection of Anti-Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis[J]. J Clin Microbiol, 2016, 54(11): 2661-2668.
[23]
Rish JA, Eisenach KD, Cave MD, et al. Polymerase chain reaction detection of Mycobacterium tuberculosis in formalin-fixed tissue[J]. Am J Respir Crit Care Med, 1996, 153(4 Pt 1): 1419-1423.
[24]
Chen L, Liu Z, Su Y, et al. Characterization of Mycobacterium marinum infections in zebrafish wounds and sinus tracts[J]. Wound Repair Regen, 2017, 25(3): 536-540.
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