探讨低渗丙酮酸钠口服补液盐对大鼠窒息性心脏停搏复苏后脑损伤的影响。

清洁级雄性SD大鼠，体重300～350 g，按照随机数字表法分为3组：假手术组(S组，n＝8)、窒息性心脏停搏复苏＋实验用水组(C组，n＝12)和窒息性心脏停搏复苏＋低渗丙酮酸钠口服补液盐组(P组，n＝12)。P组于大鼠复苏后30 min经口胃管置入法灌注37 ℃低渗丙酮酸钠口服补液盐12 mL(3 mL/次，每间隔30 min灌注1次)，随后拔除胃管放回笼中，首日以低渗丙酮酸钠口服补液盐自由饮用。C组于大鼠复苏后30 min用同样的方法灌注等量的实验用水，随后拔除胃管放回笼中，自由饮用实验用水。S组不诱导窒息性心脏停搏及心肺复苏。复苏后24 h取大脑额叶皮层组织测丙酮酸含量。复苏后24、72 h和复苏后7 d时行神经功能缺损评分。复苏后4～7 d进行Morris水迷宫实验检测空间学习记忆能力。复苏后7 d观察海马CA1区神经元存活情况。

与S组比较，C组脑组织丙酮酸含量降低，而P组的含量显著增高；C组和P组各时间点神经缺损评分与海马CA1区存活神经元数量降低，水迷宫实验显示逃避潜伏期延长、平台所在象限进入百分比和平台象限停留时间百分比都降低，差异均有统计学意义(P值均小于0.05)。但与C组比较，P组各时间点神经缺损评分与海马CA1区存活神经元数量升高；逃避潜伏期缩短、平台所在象限进入百分比和平台象限停留时间百分比都明显升高，差异均有统计学意义(P值均小于0.05)。

低渗丙酮酸钠口服补液盐可以减轻大鼠窒息性心脏停博复苏后脑损伤。

To investigate the effect of hypotonic pyruvate oral rehydration solution on brain injury subjected asphyxial cardiac arrest in rats.

Clean male SD rats, weighing 300-350 g, were divided into 3 groups according to random number table method: sham operation group (group S, n=8), asphyxial cardiac arrest recovery plus experimental water group (group C, n=12) and asphyxial cardiac arrest recovery plus hypotonic pyruvate oral rehydration solution group (group P, n=12). At 30 min after the restoration of spontaneous circulation, group P was gastrically injected through gavage of pyruvate oral rehydration solution 12 mL, instead of experimental water in group C(3 mL at 30 min intervals), then drinking pyruvate oral rehydration solution to replace experimental water in the first day. While rats in group S were not induced asphyxial cardiac arrest and cardiopulmonary resuscitation. Pyruvate levels of brain frontal cortex was measured at 24 h after restoration of spontaneous circulation. The scores of neurological deficits were evaluated 24 h, 72 h and 7 d following the cardiac arrest restoration. The water maze tests used to evaluate spatial learning and memory deficit were recorded 4-7 d post-restoration of spontaneous circulation. Morphological changes of neurons in the hippocampal CA1 region were observed on 7 d after the cardiac arrest recovery.

Compared with group S, brain pyruvate levels in group C were decreased, but greatly increased in group P, in groups C and P, the neurological deficit score and the number of viable neurons in hippocampal CA1 region were reduced, the escape latency was prolonged, and the platform quadrant enter time percentage and the target quadrant residence time percentage were decreased at each time point, the differences were statistically significant(with P values below 0.05). However, compared with group C, in group P with increased pyruvate in the brain tissue, the score of nerve defect and the number of survival neurons in hippocampal CA1 region were significantly improved, the escape latency was shortened, and the two percentages of the platform quadrant were also preserved at each time point, the differences were statistically significant(with P values below 0.05).

Oral hypotonic pyruvate oral rehydration solution relieved the brain damage after asphyxial cardiac arrest in rats.