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中华损伤与修复杂志(电子版) ›› 2019, Vol. 14 ›› Issue (05) : 388 -392. doi: 10.3877/cma.j.issn.1673-9450.2019.05.014

所属专题: 文献

综述

细胞悬液移植技术在创面修复中的研究与应用进展
姜耀男1, 肖仕初1,()   
  1. 1. 200433 海军军医大学第一附属医院烧伤外科
  • 收稿日期:2019-08-08 出版日期:2019-10-01
  • 通信作者: 肖仕初
  • 基金资助:
    国家自然科学基金面上项目(81871559)

Progress in research and application of cell suspension transplantation technology in wound repair

Yaonan Jiang1, Shichu Xiao1,()   

  1. 1. Department of Burns Surgery, First Affiliated Hospital, Naval Military Medical University, Shanghai 200433, China
  • Received:2019-08-08 Published:2019-10-01
  • Corresponding author: Shichu Xiao
  • About author:
    Corresponding author: Xiao Shichu, Email:
引用本文:

姜耀男, 肖仕初. 细胞悬液移植技术在创面修复中的研究与应用进展[J]. 中华损伤与修复杂志(电子版), 2019, 14(05): 388-392.

Yaonan Jiang, Shichu Xiao. Progress in research and application of cell suspension transplantation technology in wound repair[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2019, 14(05): 388-392.

由各类因素所导致的急性、慢性、难愈性及医源性创面在临床上十分常见,常严重影响患者的生活质量并增加患者经济及社会医疗负担。随着细胞悬液移植相关的制备技术和载体材料研究趋渐成熟,其降低供皮区面积、治疗效果明确等优点使得自体/异体细胞应用于皮肤创面的治疗研究成为近年来创面修复临床研究的热点之一。但细胞悬液制备技术和载体技术目前尚无统一标准且部分技术尚未进行严格临床研究验证,仍需进行进一步临床研究明确其疗效及适应证。本文从细胞悬液的制备、移植及临床应用3个方面,对细胞悬液移植技术在创面修复方面的研究与应用进行归纳整理,以期为临床及科研工作者提供参考依据。

Acute, chronic, refractory and iatrogenic wounds caused by various factors are very commonn in the clinic, often seriously affecting the quality of life of patients and increasing the burden of economy and social medical resources. With the progress of preparation technology and carrier materials related to cell suspension transplantation, it has the advantages of reducing the area of donor site and clear therapeutic effect, making the treatment of autologous/allogeneic cells applied to skin wounds become the clinical research hotspots of wound repair in recent years. However, there is no uniform standard for cell suspension preparation technology and carrier technology, and some technologies have not been verified by rigorous clinical research. Further clinical research is needed to clarify its efficacy and indications. This article reviewes the research and application of cell suspension transplantation technology in wound repair on the 3 aspects of preparation, transplantation and clinical application of cell suspension, in order to provide reference for clinicians and scientific researchers.

[1]
Ter Horst B, Chouhan G, Moiemen NS, et al. Advances in keratinocyte delivery in burn wound care[J]. Adv Drug Deliv Rev, 2018, 123: 18-32.
[2]
Wood FM, Giles N, Stevenson A, et al. Characterisation of the cell suspension harvested from the dermal epidermal junction using a ReCell® kit[J]. Burns, 2012, 38(1): 44-51.
[3]
Mcheik JN, Barrault C, Levard G, et al. Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective[J]. Plast Reconstr Surg Glob Open, 2014, 2(9): e218.
[4]
Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells[J]. Cell, 1975, 6(3): 331-343.
[5]
Kirsner RS, Vanscheidt W, Keast DH, et al. Phase 3 evaluation of HP802-247 in the treatment of chronic venous leg ulcers[J]. Wound Repair Regen, 2016, 24(5): 894-903.
[6]
杨晓龙,巴特,刘玲英. 间充质干细胞促进慢性创面血管新生的机制及应用[J/CD]. 中华损伤与修复杂志(电子版), 2015, 10(1): 76-79.
[7]
杨靖,柴家科,刘玲英. 间充质干细胞对烧伤创面免疫调控作用的研究进展[J/CD]. 中华损伤与修复杂志(电子版), 2014, 9(6): 668-671.
[8]
Rodgers K, Jadhav SS. The application of mesenchymal stem cells to treat thermal and radiation burns[J]. Adv Drug Deliv Rev, 2018, 123: 75-81.
[9]
Mansilla E, Marín GH, Berges M, et al. Cadaveric bone marrow mesenchymal stem cells: first experience treating a patient with large severe burns[J]. Burns Trauma, 2015, 3: 17.
[10]
The ReCell Spray-On Skin system for treating skin loss, scarring and depigmentation after burn injury[EB/OL].[2014-11].

URL    
[11]
Gerlach JC, Johnen C, Ottomann C, et al. Method for autologous single skin cell isolation for regenerative cell spray transplantation with non-cultured cells[J]. Int J Artif Organs, 2011, 34(3): 271-279.
[12]
Esteban-Vives R, Young MT, Zhu T, et al. Calculations for reproducible autologous skin cell-spray grafting[J]. Burns, 2016, 42(8): 1756-1765.
[13]
Esteban-Vives R, Corcos A, Choi MS, et al. Cell-spray auto-grafting technology for deep partial-thickness burns: Problems and solutions during clinical implementation[J]. Burns, 2018, 44(3): 549-559.
[14]
Holla AP, Sahni K, Kumar R, et al. Acral vitiligo and lesions over joints treated with non-cultured epidermal cell suspension transplantation[J]. Clin Exp Dermatol, 2013, 38(4): 332-337.
[15]
Sarangal R, Yadav S, Sakral A, et al. Noncultured epidermal-melanocyte cell suspension and dermal-fat grafting for the reconstruction of an irregular, atrophic, and depigmented forehead scar: an innovative approach[J]. J Cosmet Dermatol, 2015, 14(4): 332-335.
[16]
Seghers AC, Goh BK, Tan SH, et al. Simplified noncultured autologous cell grafting for the treatment of chronic nonhealing ulcers: the six-well plate technique[J]. Clin Exp Dermatol, 2014, 39(5): 620-623.
[17]
Kumar R, Parsad D, Singh C, et al. Four compartment method: a simplified and cost-effective method of noncultured epidermal cell suspension for the treatment of vitiligo[J]. Br J Dermatol, 2014, 170(3): 581-585.
[18]
Gupta S, Sahni K, Tembhre MK, et al. A novel point-of-care in vivo technique for preparation of epidermal cell suspension for transplantation in vitiligo[J]. J Am Acad Dermatol, 2015, 72(2): e65-66.
[19]
Yoon C, Lee J, Jeong H, et al. Rapid preparation of a noncultured skin cell suspension that promotes wound healing[J]. Cell Tissue Bank, 2017, 18(2): 131-141.
[20]
Navarro FA, Stoner ML, Park CS, et al. Sprayed keratinocyte suspensions accelerate epidermal coverage in a porcine microwound model[J]. J Burn Care Rehabil, 2000, 21(6): 513-518.
[21]
Fredriksson C, Kratz G, Huss F. Transplantation of cultured human keratinocytes in single cell suspension: a comparative in vitro study of different application techniques[J]. Burns, 2008, 34(2): 212-219.
[22]
Weiss RA. Autologous cell therapy: will it replace dermal fillers?[J]. Facial Plast Surg Clin North Am, 2013, 21(2): 299-304.
[23]
Gualeni B, Coulman SA, Shah D, et al. Minimally invasive and targeted therapeutic cell delivery to the skin using microneedle devices[J]. Br J Dermatol, 2018, 178(3): 731-739.
[24]
Mansilla E, Marín GH, Drago H, et al. Bloodstream cells phenotypically identical to human mesenchymal bone marrow stem cells circulate in large amounts under the influence of acute large skin damage: new evidence for their use in regenerative medicine[J]. Transplant Proc, 2006, 38(3): 967-969.
[25]
Lee H. Outcomes of sprayed cultured epithelial autografts for full-thickness wounds: a single-centre experience[J]. Burns, 2012, 38(6): 931-936.
[26]
Grant I, Warwick K, Marshall J, et al. The co-application of sprayed cultured autologous keratinocytes and autologous fibrin sealant in a porcine wound model[J]. Br J Plast Surg, 2002, 55(3): 219-227.
[27]
Johnstone P, Kwei JS, Filobbos G, et al. Successful application of keratinocyte suspension using autologous fibrin spray[J]. Burns, 2017, 43(3): e27-e30.
[28]
Harkin DG, Dawson RA, Upton Z. Optimized delivery of skin keratinocytes by aerosolization and suspension in fibrin tissue adhesive[J]. Wound Repair Regen, 2006, 14(3): 354-363.
[29]
Zweifel CJ, Contaldo C, Köhler C, et al. Initial experiences using non-cultured autologous keratinocyte suspension for burn wound closure[J]. J Plast Reconstr Aesthet Surg, 2008, 61(11): e1-4.
[30]
Hartmann B, Ekkernkamp A, Johnen C, et al. Sprayed cultured epithelial autografts for deep dermal burns of the face and neck[J]. Ann Plast Surg, 2007, 58(1): 70-73.
[31]
Valerio IL, Hammer DA, Rendon JL, et al. A Case Report of the First Nonburn-related Military Trauma Victim Treated with Spray Skin Regenerative Therapy in Combination with a Dermal Regenerate Template[J]. Plast Reconstr Surg Glob Open, 2016, 4(12): e1174.
[32]
Hu ZC, Chen D, Guo D, et al. Randomized clinical trial of autologous skin cell suspension combined with skin grafting for chronic wounds[J]. Br J Surg, 2015, 102(2): e117-123.
[33]
Hu Z, Guo D, Liu P, et al. Randomized clinical trial of autologous skin cell suspension for accelerating re-epithelialization of split-thickness donor sites[J]. Br J Surg, 2017, 104(7): 836-842.
[34]
Kumar P, Bhari N, Tembhre MK, et al. Study of efficacy and safety of noncultured, extracted follicular outer root sheath cell suspension transplantation in the management of stable vitiligo[J]. Int J Dermatol, 2018, 57(2): 245-249.
[35]
O′Neill TB, Rawlins J, Rea S, et al. Treatment of a large congenital melanocytic nevus with dermabrasion and autologous cell suspension (ReCELL®): a case report[J]. J Plast Reconstr Aesthet Surg, 2011, 64(12): 1672-1676.
[36]
Gravante G, Di Fede MC, Araco A, et al. A randomized trial comparing ReCell system of epidermal cells delivery versus classic skin grafts for the treatment of deep partial thickness burns[J]. Burns, 2007, 33(8): 966-972.
[37]
Wood FM, Kolybaba ML, Allen P. The use of cultured epithelial autograft in the treatment of major burn wounds: eleven years of clinical experience[J]. Burns, 2006, 32(5): 538-544.
[38]
Kym D, Yim H, Yoon J, et al. The application of cultured epithelial autografts improves survival in burns[J]. Wound Repair Regen, 2015, 23(3): 340-344.
[39]
James SE, Booth S, Dheansa B, et al. Sprayed cultured autologous keratinocytes used alone or in combination with meshed autografts to accelerate wound closure in difficult-to-heal burns patients[J]. Burns, 2010, 36(3): e10-20.
[40]
Jackson PC, Wilks D, Rawlins J, et al. Combined use of hyperbaric oxygen and sprayed keratinocyte suspension to tackle a difficult wound[J]. Ann R Coll Surg Engl, 2014, 96(6): e20-22.
[41]
Y K, B R K, TD, et al. Treatment of rhinophyma with the Versajet™ Hydrosurgery System and autologous cell suspension (ReCELL®): A case report[J]. J Cosmet Laser Ther, 2018, 20(2): 114-116.
[42]
Busch KH, Bender R, Walezko N, et al. Combination of medical needling and non-cultured autologous skin cell transplantation (ReNovaCell) for repigmentation of hypopigmented burn scars[J]. Burns, 2016, 42(7): 1556-1566.
[43]
Migliano E, Bellei B, Govoni FA, et al. Fat and epidermal cell suspension grafting: a new advanced one-step skin regeneration surgical technique[J]. J Exp Clin Cancer Res, 2014, 33: 23.
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