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中华损伤与修复杂志(电子版) ›› 2024, Vol. 19 ›› Issue (02) : 113 -118. doi: 10.3877/cma.j.issn.1673-9450.2024.02.004

电烧伤

高压电烧伤大鼠肝脏氧化应激损伤及灯盏花素的干预作用
鲁梦远1, 赵学刚1, 郝嘉文1, 盖晨阳1, 李聪颖1, 张晶1, 张庆富1,()   
  1. 1. 050035 石家庄,河北医科大学第三医院烧伤与创面修复中心 河北省烧伤救治技术创新中心
  • 收稿日期:2024-01-31 出版日期:2024-04-01
  • 通信作者: 张庆富
  • 基金资助:
    河北省创新能力提升计划项目(225676120H)

Hepatic oxidative stress injury in high-voltage electrical burns rats and the intervention effect of brevisanthin

Mengyuan Lu1, Xuegang Zhao1, Jiawen Hao1, Chenyang Gai1, Congying Li1, Jing Zhang1, Qingfu Zhang1,()   

  1. 1. Burn and Wound Repair Center, The Third Hospital of Hebei Medical University, Hebei Burn Treatment Technology Innovation Center, Shijiazhuang 050035, China
  • Received:2024-01-31 Published:2024-04-01
  • Corresponding author: Qingfu Zhang
引用本文:

鲁梦远, 赵学刚, 郝嘉文, 盖晨阳, 李聪颖, 张晶, 张庆富. 高压电烧伤大鼠肝脏氧化应激损伤及灯盏花素的干预作用[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(02): 113-118.

Mengyuan Lu, Xuegang Zhao, Jiawen Hao, Chenyang Gai, Congying Li, Jing Zhang, Qingfu Zhang. Hepatic oxidative stress injury in high-voltage electrical burns rats and the intervention effect of brevisanthin[J/OL]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2024, 19(02): 113-118.

目的

探讨氧化应激在大鼠高压电烧伤肝损伤过程中的作用及灯盏花素的干预效果。

方法

本研究分为电伤实验和治疗实验两部分。采用随机数字表法将170只雄性SD大鼠分配至电伤实验(120只)和治疗实验(50只)。将电伤实验分为电伤组(60只)和假伤组(60只),观测时相为电伤后0、8、24、48、72 h及1周,每个观测时相10只。将治疗实验分为高、中、低剂量组,电伤组和盐水组,每组10只。采用实验高压电击系统制作电压3 kV、电击3 s的大鼠高压电烧伤模型。电伤实验大鼠按时相采集血清及肝脏组织。治疗实验大鼠高、中、低剂量组分别腹腔注射6 mg/kg、4 mg/kg和2 mg/kg灯盏花素,盐水组腹腔注射生理盐水1mL/kg,电伤组作空白对照,治疗72 h后处死并采集血清及肝脏组织。光镜下观察肝组织形态学变化,检测血清中总胆红素(TBIL)含量,谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,检测肝脏匀浆上清中还原型谷胱甘肽(GSH)、超氧阴离子(O2-·)、总巯基(-SH)含量,黄嘌呤氧化酶(XOD)活性及羟自由基(·OH)清除率。

结果

高压电烧伤大鼠肝组织进行性微循环障碍及炎性细胞浸润,使用灯盏花素治疗后肝损伤减轻。电伤实验中,电伤组TBIL、O2-·含量和·OH清除率与假伤组比较,差异无统计学意义(F=0.184、0.485、0.119, P=0.678、0.504、0.739);电伤组ALT、AST和XOD活性高于假伤组,差异有统计学意义(F=215.500、92.010、65.400, P<0.001);电伤组-SH和GSH含量低于假伤组,差异有统计学意义(F=27.890、113.500, P<0.001)。治疗实验组间相互比较,TBIL、O2-·含量和·OH清除率差异无统计学意义(F=1.441、1.890、0.249,P=0.236、0.129、0.909);与电伤组和盐水组比较,中剂量组ALT、AST、XOD活性和-SH含量降低,GSH含量升高,差异有统计学意义(P<0.05)。

结论

高压电烧伤导致大鼠肝脏组织和细胞损伤,可能与氧化应激相关。灯盏花素对高压电烧伤大鼠肝脏损伤和氧化应激有改善作用。

Objective

To investigate the role of oxidative stress in liver injury caused by high-voltage electrical burns in rats and the intervention effect of brevisanthin.

Methods

The study was divided into electrotrauma experiment and treatment experiment. A total of 170 male SD rats were assigned to the electrotrauma experiment (n=120) and the treatment experiment (n=50) using the random number table method. The electrotrauma experiment was divided into electrical injury group (n=60) and sham electrical injury group (n=60), and the observation phases were 0, 8, 24, 48, 72 h and 1 week after electrical injury, and 10 animals were observed in each observation phase. The treatment experiment was divided into high-dose, medium-dose and low-dose treatment groups, electrical injury group and saline group, with 10 animals in each group. The experimental high-voltage electrical shock system was used to make high-voltage electrical burn model of rats with a voltage of 3 kV and an electric shock of 3 seconds. Electrotrauma experimental rats were collected serum and liver tissue on time. The high-dose, medium-dose, and low-dose groups were intraperitoneally injected with 6 mg/kg, 4 mg/kg, and 2 mg/kg brevisanthin, respectively, the saline group was intraperitoneally injected with 1 mL/kg of normal saline, and the electrical injury group was used as blank control, and the serum and liver tissue were sacrificed and collected after 72 hours of treatment. The morphological changes of liver tissue were observed under light microscopy, the content of total bilirubin (TBIL) and glutamic-pyruvic transaminase (ALT), glutamic-oxaloacetic transaminase (AST) activity in serum were measured, and the contents of glutathione (GSH), superoxide radical (O2-·) and total sulfhydryl (-SH), xanthine oxidase (XOD) activity and hydroxyl radical (·OH) scavenging rate in the supernatant of liver homogenate were detected.

Results

Progressive microcirculatory disorders and inflammatory cell infiltration occurred in liver tissue of high-voltage electrical burns rats, liver damage was reduced after treatment with brevisanthin. In the electrotrauma experiment, there was no significant difference in the content of TBIL, O2-·and ·OH scavenging rate between the electrical injury group and the sham electrical group(F=0.184, 0.485, 0.119; P=0.678, 0.504, 0.739). The ALT, AST, XOD activity in the electrical group were higher than those in the sham electrical group, the difference was significant (F=215.500, 92.010, 65.400, P<0.001). The content of GSH and -SH in the electrical injury group were lower than those in the sham electrical group, and the difference was significant (F=27.890, 113.500, P<0.001). There was no significant difference in the content of TBIL, O2-· and ·OH scavenging rate among treatment experimental groups (F=1.441, 1.890, 0.249; P=0.236, 0.129, 0.909). The ALT, AST, XOD activity and the content of -SH in the middle-dose group decreased and the content of GSH increased, and the difference was significant(P<0.05).

Conclusion

High-voltage electrical burns cause liver tissue and cell damage in rats, which may be associated with oxidative stress. Brevisanthin has an ameliorating effect on liver injury and oxidative stress in rats with high-voltage electrical burns.

图1 电伤实验中部分时相大鼠肝脏的病理变化(HE染色)。A-D分别示假伤组及电伤组伤后24、72 h及1周时10倍镜下所见,肝窦间隙逐渐缩窄、消失;E-H分别示假伤组及电伤组伤后24、72 h及1周时40倍镜下所见,炎细胞浸润、黏附,微血管先扩张,之后随时间推移狭窄闭塞
图2 电伤实验不同时相大鼠肝脏损伤与氧化应激指标的变化。A-H分别示电伤组和假伤组不同时相TBIL、ALT、AST、XOD、O2-·、·OH、-SH及GSH的变化。a示组间比较差异有统计学意义(P<0.05);b、c、d分别示与伤后8、24、48 h比较,差异有统计学意义(P<0.05)
图3 治疗实验中部分处理方式下大鼠肝脏的病理变化(HE染色)。A-E分别示电伤组、盐水组、低剂量组、中剂量组和高剂量组10倍镜下所见,与A、B比较,C、D、E图中肝窦间隙的血液沉积、排布杂乱情况显著改善;F-J分别示电伤组、盐水组、低剂量组、中剂量组和高剂量组40倍镜下所见,与F、G比较,H、I、J图中炎细胞及血液沉积减少,肝细胞排列规则,胞核质均
图4 治疗实验中不同处理方式下肝脏损伤与氧化应激指标的变化。A-H分别示各组TBIL、ALT、AST、XOD、O2-·、·OH、-SH、GSH变化。a示与电伤组比较差异有统计学意义(P<0.05);b示与盐水组比较差异有统计学意义(P<0.05)
[1]
孙永华. 我国电烧伤治疗的成就与挑战[J]. 中华烧伤杂志200824(5):381-383.
[2]
Ding H, Huang M, Li D,et al. Epidemiology of electrical burns: a 10-year retrospective analysis of 376 cases at a burn centre in South China[J]. J Int Med Res, 202048(3):300060519891325.
[3]
张庆富. 关注电烧伤所致精神障碍[J]. 中华烧伤杂志202036(6):415-418.
[4]
张庆富,张景,张海华.实验高压电烧伤后肝脏微循环动态变化及意义[J].中国微循环200812(2):97-100,131.
[5]
Ji LL, Yeo D. Oxidative stress: an evolving definition[J]. Fac Rev202110:13.
[6]
赵茜,罗德霞,马琼,等.基于血小板活化因子研究灯盏花素改善非酒精性脂肪肝病大鼠氧化应激作用[J].中华中医药杂志202237(1):461-464.
[7]
司海莉. 高压电烧伤对大鼠血清ROS和GSH的影响及乌司他丁的干预作用[D].河北医科大学,2018.
[8]
李梅秀.高压电烧伤对大鼠胰腺SOD、iNOS、血糖的影响及NAC的干预作用[D].河北医科大学,2023.
[9]
张庆富,郝嘉文. 高压电烧伤进行性损伤的机制及防治策略[J]. 中华烧伤与创面修复杂志202339(8):718-723.
[10]
张庆富.微循环治疗技术在烧伤临床中的应用[J/CD].中华损伤与修复杂志(电子版)201712(2):90-93.
[11]
Batista Napotnik T, Polajžer T, Miklavčič D . Cell death due to electroporation - a review[J]. Bioelectrochemistry, 2021, 141:107871.
[12]
张庆富,魏伟,尚涛,等. 高压电烧伤对大鼠血小板与白细胞流变性的影响及己酮可可碱的干预作用[J]. 中华烧伤杂志201228(6):428-434.
[13]
Michel O, Pakhomov AG, Casciola M, et al. Electropermeabilization does not correlate with plasma membrane lipid oxidation[J]. Bioelectrochemistry, 2020132:107433.
[14]
Szlasa W, Kiełbik A, Szewczyk A, et al. Oxidative effects during irreversible electroporation of melanoma cells-in vitro study[J]. Molecules202026(1):154.
[15]
张庆富,许顺江,梁利民,等.高压电烧伤对大鼠小肠浆膜表面微循环灌流量的影响及己酮可可碱的干预作用[J].中华烧伤杂志201733(3):166-170.
[16]
徐列明.改善肝脏微循环障碍的必要性和相关中药的筛选[J].临床肝胆病杂志201329(4):257-259,263.
[17]
Perez A, Kortgen A, Leonhardt J, et al. Critical care hepatology: definitions, incidence, prognosis and role of liver failure in critically ill patients[J]. Crit Care202226(1):289.
[18]
Collin F. Chemical basis of reactive oxygen species reactivity and involvement in neurodegenerative diseases[J]. Int J Mol Sci201920(10):2407.
[19]
Jomova K, Raptova R, Alomar SY, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging[J]. Arch Toxicol202397(10):2499-2574.
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