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中华损伤与修复杂志(电子版) ›› 2021, Vol. 16 ›› Issue (01) : 1 -5. doi: 10.3877/cma.j.issn.1673-9450.2021.01.001

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间充质干细胞源性外泌体在促创面愈合中的应用与挑战
祁放1, 王达利1,()   
  1. 1. 563003 遵义医科大学附属医院烧伤整形外科
  • 收稿日期:2021-01-10 出版日期:2021-02-01
  • 通信作者: 王达利
  • 基金资助:
    国家自然科学基金(81871570,82072195); 贵州省科技计划项目(黔科合支撑【2016】2910号); 贵州省科技计划项目(黔科合支撑【2020】4Y148号); 贵州省组织损伤修复与再生医学2011协同创新中心(黔教合协同创新字【2015】07)

Application and challenges of exosomes drived from mesenchymal stem cells in wound healing

Fang Qi1, Dali Wang1,()   

  1. 1. Department of Burns and Plastic Surgery, First Affiliated Hospital of Zunyi Medical University, Zunyi 563003, China
  • Received:2021-01-10 Published:2021-02-01
  • Corresponding author: Dali Wang
引用本文:

祁放, 王达利. 间充质干细胞源性外泌体在促创面愈合中的应用与挑战[J]. 中华损伤与修复杂志(电子版), 2021, 16(01): 1-5.

Fang Qi, Dali Wang. Application and challenges of exosomes drived from mesenchymal stem cells in wound healing[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(01): 1-5.

间充质干细胞(MSC)能够有效促进各类创面的愈合且已进入临床试验阶段。进一步研究发现MSC通过旁分泌产生的外泌体也具有与MSC等效的生物学作用。基于MSC源性外泌体的"无细胞"疗法具有广阔的应用前景,但其面临的争议与挑战也值得关注。本文就MSC及其源性外泌体的应用进展和争议,以及微环境对其的影响进行简要评述,与读者共同探讨。

Mesenchymal stem cells (MSC) can effectively promote the healing of various wounds and have reached the stage of clinical trials. Further studies have found that exosomes secreted by MSC by paracrine mechanism also have biological effects equivalent to MSC. "Cell-free" therapy based on exosomes drived from MSC has broad application prospects, but the controversy and challenges it faces are also worthy of attention. This paper briefly reviewed the application and existing problems of MSC and exosomes drived from MSC, furthermore, the influence of microenvironment was analyzed and discussed.

1
Devine SM, Bartholomew AM, Mahmud N, et al. Mesenchymal stem cells are capable of homing to the bone marrow of non-human primates following systemic infusion[J]. Exp Hematol, 2001, 29(2): 244-255.
2
Hoogduijn MJ, Roemeling-Van Rhijn M, Korevaar SS, et al. Immunological aspects of allogeneic and autologous mesenchymal stem cell therapies[J]. Hum Gene Ther, 2011, 22(12): 1587-1591.
3
Burlacu A. Tracking the mesenchymal stem cell fate after transplantation into the infarcted myocardium[J]. Curr Stem Cell Res Ther, 2013, 8(4): 284-291.
4
Noiseux N, Gnecchi M, Lopez-Ilasaca M, et al. Mesenchymal stem cells overexpressing Akt dramatically repair infarcted myocardium and improve cardiac function despite infrequent cellular fusion or differentiation[J]. Mol Ther, 2006, 14(6): 840-850.
5
Tsatsaronis JA, Franch-Arroyo S, Resch U, et al. Extracellular Vesicle RNA: A Universal Mediator of Microbial Communication?[J]. Trends Microbiol, 2018, 26(5): 401-410.
6
Wu P, Zhang B, Shi H, et al. MSC-exosome: A novel cell-free therapy for cutaneous regeneration[J]. Cytotherapy, 2018, 20(3): 291-301.
7
Uccelli A, Moretta L, Pistoia V. Mesenchymal stem cells in health and disease[J]. Nat Rev Immunol, 2008, 8(9): 726-736.
8
Caplan AI. Mesenchymal Stem Cells: Time to Change the Name![J]. Stem Cells Transl Med, 2017, 6(6): 1445-1451.
9
Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement[J]. Cytotherapy, 2006, 8(4): 315-317.
10
Bianco P. "Mesenchymal" stem cells[J]. Annu Rev Cell Dev Biol, 2014, 30: 677-704.
11
Lee DE, Ayoub N, Agrawal DK. Mesenchymal stem cells and cutaneous wound healing: novel methods to increase cell delivery and therapeutic efficacy[J]. Stem Cell Res Ther, 2016, 7: 37.
12
Kucharzewski M, Rojczyk E, Wilemska-Kucharzewska K, et al. Novel trends in application of stem cells in skin wound healing[J]. Eur J Pharmacol, 2019, 843: 307-315.
13
Galipeau J, Sensébé L. Mesenchymal Stromal Cells: Clinical Challenges and Therapeutic Opportunities[J]. Cell Stem Cell, 2018, 22(6): 824-833.
14
Kouhkheil R, Fridoni M, Piryaei A, et al. The effect of combined pulsed wave low-level laser therapy and mesenchymal stem cell-conditioned medium on the healing of an infected wound with methicillin-resistant Staphylococcal aureus in diabetic rats[J]. J Cell Biochem, 2018, 119(7): 5788-5797.
15
D′souza-Schorey C, Schorey JS. Regulation and mechanisms of extracellular vesicle biogenesis and secretion[J]. Essays Biochem, 2018, 62(2): 125-133.
16
Ma ZJ, Yang JJ, Lu YB, et al. Mesenchymal stem cell-derived exosomes: Toward cell-free therapeutic strategies in regenerative medicine[J]. World J Stem Cells, 2020, 12(8): 814-840.
17
Hu P, Yang Q, Wang Q, et al. Mesenchymal stromal cells-exosomes: a promising cell-free therapeutic tool for wound healing and cutaneous regeneration[J]. Burns Trauma, 2019, 7: 38.
18
Zhang Y, Bi J, Huang J, et al. Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications[J]. Int J Nanomedicine, 2020, 15: 6917-6934.
19
Tang YT, Huang YY, Zheng L, et al. Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum[J]. Int J Mol Med, 2017, 40(3): 834-844.
20
Ding M, Wang C, Lu X, et al. Comparison of commercial exosome isolation kits for circulating exosomal microRNA profiling[J]. Anal Bioanal Chem, 2018, 410(16): 3805-3814.
21
Ludwig N, Whiteside TL, Reichert TE. Challenges in Exosome Isolation and Analysis in Health and Disease[J]. Int J Mol Sci, 2019, 20(19): 4684.
22
Gardiner C, Di Vizio D, Sahoo S, et al. Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey[J]. J Extracell Vesicles, 2016, 5: 32945.
23
Yang D, Zhang W, Zhang H, et al. Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics[J]. Theranostics, 2020, 10(8): 3684-3707.
24
Li P, Kaslan M, Lee SH, et al. Progress in Exosome Isolation Techniques[J]. Theranostics, 2017, 7(3): 789-804.
25
Théry C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines[J]. J Extracell Vesicles, 2018, 7(1): 1535750.
26
白金权,李柏文,鲁晶,等. 肝癌干细胞源性外泌体对肝癌细胞增殖耐药性的影响[J]. 吉林医药学院学报,2021, 42(1): 5-9.
27
Kalluri R, Lebleu VS. The biology, function, and biomedical applications of exosomes[J]. Science, 2020, 367(6478): eaau6977.
28
Chevillet JR, Kang Q, Ruf IK, et al. Quantitative and stoichiometric analysis of the microRNA content of exosomes[J]. Proc Natl Acad Sci U S A, 2014, 111(41): 14888-14893.
29
曾良,万朝辉,周辉. miR-22修饰的骨髓间充质干细胞外泌体抑制急性心肌梗死大鼠心肌细胞凋亡[J]. 中国急救医学,2021, 41(2): 154-160.
30
刘梦瑶,姜立新. 外泌体活体示踪的分子影像学研究进展[J]. 医学研究杂志,2021, 50(1): 145-148.
31
Sun Z, Shi K, Yang S, et al. Effect of exosomal miRNA on cancer biology and clinical applications[J]. Mol Cancer, 2018, 17(1): 147.
32
Pegtel DM, Cosmopoulos K, Thorley-Lawson DA, et al. Functional delivery of viral miRNAs via exosomes[J]. Proc Natl Acad Sci U S A, 2010, 107(14): 6328-6333.
33
Barile L, Vassalli G. Exosomes: Therapy delivery tools and biomarkers of diseases[J]. Pharmacol Ther, 2017, 174: 63-78.
34
Phinney DG, Pittenger MF. Concise Review: MSC-Derived Exosomes for Cell-Free Therapy[J]. Stem Cells, 2017, 35(4): 851-858.
35
Kusuma GD, Carthew J, Lim R, et al. Effect of the Microenvironment on Mesenchymal Stem Cell Paracrine Signaling: Opportunities to Engineer the Therapeutic Effect[J]. Stem Cells Dev, 2017, 26(9): 617-631.
36
Wang L, Hu L, Zhou X, et al. Author Correction: Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling[J]. Sci Rep, 2018, 8(1): 7066.
37
Ho IA, Toh HC, Ng WH, et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis[J]. Stem Cells, 2013, 31(1): 146-155.
38
Lee JK, Park SR, Jung BK, et al. Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells[J]. PLoS One, 2013, 8(12): e84256.
39
杨翔宇,李晓红,肖静,等. 干扰素γ刺激hUC-MSCs分泌外泌体促进调节性T细胞生成[J]. 中国药理学通报,2017, 33(1): 45-51.
40
Ti D, Hao H, Tong C, et al. LPS-preconditioned mesenchymal stromal cells modify macrophage polarization for resolution of chronic inflammation via exosome-shuttled let-7b[J]. J Transl Med, 2015, 13: 308.
41
Madrigal M, Rao KS, Riordan NH. A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods[J]. J Transl Med, 2014, 12: 260.
42
Yang J, Wang X, Liu S, et al. BDNF expression is up-regulated by progesterone in human umbilical cord mesenchymal stem cells[J]. Neurol Res, 2016, 38(12): 1088-1093.
43
Bustos ML, Huleihel L, Meyer EM, et al. Activation of human mesenchymal stem cells impacts their therapeutic abilities in lung injury by increasing interleukin (IL)-10 and IL-1RN levels[J]. Stem Cells Transl Med, 2013, 2(11): 884-895.
44
魏在荣,王达利. 间充质干细胞与创面修复——间充质干细胞微环境细胞外囊泡假说[J]. 中华整形外科杂志,2019, 35(4): 324-330.
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