Abstract:
Objective To study the hemostatic efficacy of macroporous polysaccharide complex hemostatic materials(MPCHM)with microporous structure applied to simulate clinic hepatic trauma model, the efficacy and safety of liver repair function of the materials was evaluted.
Methods Hepatic trauma model in clinic was mimicked. The 24 New Zealand rabbits were divided into the experimental group (using MPCHM) and the blank group (not using any hemostatic materials) according to the random number table, and the hemostatic time and amount of bleeding were recorded. The blood routine, biochemical analysis and coagulation indexes were detected after 1, 4, 8 weeks by collecting rabbit′s peripheral blood, and independent sample t test was completed by analyzing all the obtained data.
Results The hemostatic time of the experimental and blank group were (105.00±29.15)s and (381.00±54.86)s, the difference was statistically significant (t=-4.442, P<0.05). The amount of bleeding blood of the experimental and blank group were (0.45±0.26)g and (1.26±0.51)g, the difference was statistically significant (t=-14.048, P<0.05). The lymphocyte content in the experimental group in 1 and 8 weeks after operation were significantly lower than the blank group(t=-12.595, -7.909; with P values below 0.05). Alanine transaminase and aspartate aminotransferase in the experimental group in 8 weeks after operation were significantly lower than the blank group(t=-5.613, -3.656; with P values below 0.05). White blood cell count after operation were significantly higher than the blank group(t=5.521, 6.433, 2.399; with P values below 0.05). Albumin in the experimental group in 1 and 8 weeks after operation were significantly higher than the blank group(t=3.120, 5.168; with P values below 0.05). Activited partial thomboplastin time and prothrombin time in the experimental group were in normal range after operation.
Conclusions The MPCHM have good biological safety and can promote the function recovery of liver and reduce the body′s stress response to liver injury. The materials will not cause adverse body inflammatory reaction, rejection and biological toxicity during the period. The materials provide a new way for solving the problem of completely preserving liver tissue in clinical liver injury hemostasis.
Key words:
Wounds, nonpenetrating,
Liver,
Hemostasis,
Macroporous polysaccharide complex hemostatic materials,
Radiation crosslink
Lijun Zeng, Gangyi Cheng, Yun Wang, Ling Xu, Li Deng, Lianfeng Lin. Hemostatic efficacy of macroporous polysaccharide complex hemostatic materials on acute hepatic blunt trauma[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2018, 13(02): 88-93.