Preparation and properties of silver-containing mesoporous silica-chitosan composites

doi:10.3877/cma.j.issn.1673-9450.2019.04.004

Abstract

Abstract:

Objective

To investigate the preparation method of silver-containing mesoporous silica nanoparticle-chitosan(Ag/MSN-Chi) composites and their microscopic representation, cytotoxicity, water absorption, rapid hemostatic properties and antibacterial properties.

Methods

Using ethyl orthosilicate as precursor and cetyltrimethylammonium bromide as porogen, silver ions were introduced into mesoporous silica nanoparticles (MSN) by ion-exchange method to prepare novel ordered silver-containing MSN (Ag/MSN) material with antibacterial action. Ag/MSN-Chi was prepared by using alkylated chitosan loaded with Ag/MSN. The experiment were divided into experimental group and blank control group according to the materials used. The experimental group was further divided into three subgroups: MSN group, Ag/MSN group and Ag/MSN-Chi group. The blank group was a positive control without material. The specific surface area, pore volume, pore size of MSN and Ag/MSN, the charge of Ag/MSN and Ag/MSN-Chi were calculated. The cytotoxicity, water absorption performance, hemostatic and antibacterial properties of MSN, Ag/MSN and Ag/MSN-Chi were evaluated by water absorption test, in vitro coagulation test and antibacterial test to calculate the relative cell survival rate, water absorption rate and prothrombin time (PT), activated partial thromboplastin time (APTT), and bacteriostatic rate. Eighteen healthy adult New Zealand white rabbits were randomly divided into three groups: control group (treated with medical gauze), Ag/MSN group (treated with Ag/MSN), and Ag/MSN-Chi group (treated with Ag/MSN-Chi), 6 rats in each group. A hepatic trauma hemorrhage model was established and the hemostasis time was calculated. Data comparisons were analyzed by analysis of variance and t test.

Results

The specific surface area of MSN was (523.8±12.4) m²/g, the pore volume was (1.2±0.4) m³/g, and the pore diameter was (3.5±0.9) nm; the specific surface area of Ag/MSN was (521.6±11.7) m²/g, the pore volume was (1.15±0.5) m³/g, and the pore diameter was (3.6±0.7) nm, there was no significant difference in specific surface area, pore volume and pore size between the two materials (t=0.224, 0.135, 0.015; with P values above 0.05). The Zeta potential of Ag/MSN was measured to be -19.7 mV, and the Zeta potential of Ag/MSN-Chi was 10.27 mV, indicating that the surface charge of Ag/MSN changed from a negative value to a positive value. The relative survival rates of Ag/MSN-Chi group, Ag/MSN group and MSN group at 1, 4, and 7 days after co-culture with mouse myoblasts were not statistically significant (F=2.61, 4.72, 3.52; with P values above 0.05). The water absorption of Ag/MSN group was significantly different from that of MSN group and Ag/MSN-Chi group (t=0.482, 1.159; with P values above 0.05). There was no significant difference in PT between Ag/MSN-Chi group, Ag/MSN group, MSN group and blank control group (F=10.28, P>0.05). The APTT of Ag/MSN-Chi group, Ag/MSN group, MSN group and blank control group were (20.9±2.1), (28.5±3.4), (31.4±2.6), (38.7±2.5) s, respectively, the difference of the 4 groups was statistically significant (F=8.70, P<0.05); the APTT of Ag/MSN-Chi group, Ag/MSN group and MSN group were significantly different from the blank control group, the differences were statistically significant (t=9.443, 4.186, 3.506; with P values below 0.05); the difference between Ag/MSN group and Ag/MSN-Chi group of APTT was statistically significant (t=3.294, P<0.05). There was no significant difference in the inhibition rate between the MSN group at 0.5, 2, 4, 6 and 24 h (F=5.437, P>0.05); after incubation for 0.5 h, the inhibition rates of Ag/MSN group and Ag/MSN-Chi group were (99.7±5.2)% and (97.1±5.4)%, respectively, compared with the incubation rate of 0.5 h in MSN group (11.2±5.8)%, the differences were statistically significant (t=19.678, 18.775; with P values below 0.05). After 24 h of culture, the inhibition rates of Ag/MSN group and Ag/MSN-Chi group were (73.2±5.1)% and (72.9±6.9)%, respectively, compared with MSN group (11.8±5.7)%, the differences were statistically significant significance (t=13.904, 11.825; with P values below 0.05). The hemostasis time of Ag/MSN-Chi group, Ag/MSN group and control group were (12.3±1.5), (17.2±3.4), (28.1±3.8) s, respectively, the difference between the three groups was statistically significant (F=5.892, P<0.05); the Ag/MSN-Chi group and the Ag/MSN group were significantly different from the control group (t=9.473, 5.236; with P values below 0.05); and the Ag/MSN-Chi group was compared with the Ag/MSN group, the difference was statistically significant (t=3.230, P<0.05).

Conclusion

Ag/MSN-Chi has better water absorption performance, hemostatic property and antibacterial property without increasing cytotoxicity.

Key words: Chitosan, Silver, Hemostasis, Mesoporous silica nanoparticle, Antibacterial

Demeng Xia, Xuren Wang, Yuanchen Wang, Wentao Xiong, Shuogui Xu, Panyu Zhou. Preparation and properties of silver-containing mesoporous silica-chitosan composites[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2019, 14(04): 256-262.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhssyxfzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1673-9450.2019.04.004

https://zhssyxfzz.cma-cmc.com.cn/EN/Y2019/V14/I04/256

Figures/Tables 3

References 28

[1]	Björses K,Holst J. Various local hemostatic agents with different modes of action; an in vivo comparative randomized vascular surgical experimental study[J]. Eur J Vasc Endovasc Surg, 2007, 33(3): 363-370.
[2]	王胥人，桂莉，王毅欣. 美军战术战伤救护止血进展及启示[J]. 中国急救复苏与灾害医学杂志，2017, 12(8): 793-796.
[3]	Sohn VY,Eckert MJ,Martin MJ, et al. Efficacy of three topical hemostatic agents applied by medics in a lethal groin injury model[J]. J Surg Res, 2009, 154(2): 258-261.
[4]	邓乐君，樊鸿浩，李伟达，等. 可吸收止血材料的生物相容性研究进展[J]. 中国生物医学工程学报，2016, 35(2): 241-246.
[5]	李大为，何进，何凤利，等. 丝素蛋白/壳聚糖复合材料在组织工程中应用的研究进展[J]. 中国生物工程杂志，2017, 37(10): 111-117.
[6]	刘琳娜，李学拥，赵聪颖，等. 壳聚糖护创敷料用于深Ⅱ度烧伤创面的疗效和安全性：一项前瞻性、随机、单盲、阳性对照临床试验[J]. 中国组织工程研究，2017, 21(14): 2222-2226.
[7]	李正超，刘璐，张娟，等. 局部止血材料研究新进展[J]. 医疗卫生装备，2015, 36(10): 109-112.
[8]	胡何晶，段军超. 纳米二氧化硅对凝血系统的影响及研究进展[J]. 毒理学杂志，2016, 30(5): 385-389.
[9]	李晓生，刘昌胜，袁媛，等. 介孔硅基干凝胶的制备及其止血性能的研究[J]. 无机材料学报，2008, 23(2): 327-331.
[10]	Dai C,Liu C,Wei J, et al. Molecular imprinted macroporous chitosan coated mesoporous silica xerogels for hemorrhage control[J]. Biomaterials, 2010, 31(30): 7620-7630.
[11]	Wang Y,Zhao Q,Han N, et al. Mesoporous silica nanoparticles in drug delivery and biomedical applications[J]. Nanomedicine, 2015, 11(2): 313-327.
[12]	Silver S. Bacterial silver resistance: molecular biology and uses and misuses of silver compounds[J]. FEMS Microbiol Rev, 2003, 27(2/3): 341-353.
[13]	Dai C,Yuan Y,Liu C, et al. Degradable, antibacterial silver exchanged mesoporous silica spheres for hemorrhage control[J]. Biomaterials, 2009, 30(29): 5364-5375.
[14]	Jalal M,Ansari MA,Ali SG, et al. Anticandidal activity of bioinspired ZnO NPs: effect on growth, cell morphology and key virulence attributes of species[J]. Artif Cells Nanomed Biotechnol, 2018, 46(sup1): 912-925.
[15]	王美娜，李苑新，蔡兴. 羧甲基壳聚糖的性能及应用概况[J]. 中国实验方剂学杂志，2015, 21(1): 228-232.
[16]	刘璐，王淑芳，李灵芝，等. 壳聚糖/聚谷氨酸聚电解质海绵的制备及止血作用研究[J]. 中国急救复苏与灾害医学杂志，2014, 9(5): 406-410.
[17]	叶梓，王世杰，李辉，等. 血管内皮细胞中的凝血物质：理论研究新进展[J]. 中国组织工程研究，2017, 21(4): 627-632.
[18]	Cox JM,Rall JM. Evaluation of XSTAT^® and QuickClot^® Combat Gauze^® in a Swine Model of Lethal Junctional Hemorrhage in Coagulopathic Swine[J]. J Spec Oper Med, 2017, 17(3): 64-67.
[19]	Johnson D,Bates S,Nukalo S, et al. The effects of QuikClot Combat Gauze on hemorrhage control in the presence of hemodilution and hypothermia[J]. Ann Med Surg(Lond), 2014, 3(2): 21-25.
[20]	李继城，李东东，温莎莎，等. 季铵化壳聚糖止血海绵制备工艺的研究[J]. 化学推进剂与高分子材料，2017, 15(3): 68-71, 80.
[21]	Ahn SJ,Lee SJ,Kook JK, et al. Experimental antimicrobial orthodontic adhesives using nanofillers and silver nanoparticles[J]. Dent Mater, 2009, 25(2): 206-213.
[22]	余文珺，胡滨，张富强. 纳米载银无机抗菌剂对义齿基托树脂抗菌性能的影响[J]. 上海交通大学学报(医学版), 2006, 26(10): 1096-1098.
[23]	刘桐，唐慧琴，张学华，等. 镀银碳纳米管的抗菌性研究[J]. 功能材料，2007, 38(5): 802-805.
[24]	石凉，汪涛，吴大洋. 壳聚糖止血材料及最新研究进展[J]. 蚕业科学，2009, 35(4): 929-934.
[25]	卢斌，钱利强，章志量. 壳聚糖止血机理及应用进展研究[J]. 健康研究，2010, 30(1): 55-58.
[26]	尹刚，侯春林，刘万顺，等. 新型壳聚糖止血粉止血作用的实验研究[J]. 中国修复重建外科杂志，2009, 23(11): 1347-1349.
[27]	Grissom TE,Fang R. Topical hemostatic agents and dressings in the prehospital setting[J]. Curr Opin Anaesthesiol, 2015, 28(2): 210-216.
[28]	赵一璠，赵京禹，付小兵. 战创伤用局部快速止血材料的研究进展[J]. 中华创伤杂志，2018, 34(6): 534-539.

组别	样本数 (孔)	培养1 d	培养4 d	培养7 d
Ag/MSN－Chi组	3	109.0±4.3	107.0±5.2	101.0±4.9
Ag/MSN组	3	97.0±3.3	91.0±2.7	95.0±3.5
MSN组	3	103.0±3.1	109.0±4.3	102.0±4.7
F值	?	2.61	4.72	3.52
P值	?	>0.05	>0.05	>0.05

组别	样本数 (孔)	培养1 d	培养4 d	培养7 d
Ag/MSN－Chi组	3	109.0±4.3	107.0±5.2	101.0±4.9
Ag/MSN组	3	97.0±3.3	91.0±2.7	95.0±3.5
MSN组	3	103.0±3.1	109.0±4.3	102.0±4.7
F值	?	2.61	4.72	3.52
P值	?	>0.05	>0.05	>0.05

组别	样本数(孔)	APTT	PT
Ag/MSN－Chi组	3	20.9±2.1^ab	12.3±2.1
Ag/MSN组	3	28.5±3.4^a	11.9±3.5
MSN组	3	31.4±2.6^a	12.4±2.7
空白对照组	3	38.7±2.5	12.3±3.4
F值	?	8.70	10.28
P值	?	<0.05	>0.05

组别	样本数(孔)	APTT	PT
Ag/MSN－Chi组	3	20.9±2.1^ab	12.3±2.1
Ag/MSN组	3	28.5±3.4^a	11.9±3.5
MSN组	3	31.4±2.6^a	12.4±2.7
空白对照组	3	38.7±2.5	12.3±3.4
F值	?	8.70	10.28
P值	?	<0.05	>0.05

Please choose a citation manager

Content to export