Correlation analysis of nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome, inflammatory factors, cysteine aspartic acid protease-1 and prognosis of burn combined with inhalation injury

doi:10.3877/cma.j.issn.1673-9450.2020.06.002

Abstract

Abstract:

Objective

To explore the relationship between nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome, inflammatory factors, cysteine aspartic acid protease-1 (Caspase-1) and the prognosis of burn combined with inhalation injury.

Methods

The clinical data of 47 burn patients with inhalation injury from March 2015 to December 2019 in Department of Burn Surgery, Beihai People′s Hospital were analyzed retrospectively. The patients were divided into good prognosis group (n=19) and poor prognosis group (n=28) according to the recovery of disease during hospitalization. The clinical data of all patients were collected, including sex, age, hospitalization time after burns, burns area, burns depth. The levels of interleukin (IL)-1β, IL-6, IL-8, IL-18 and tumor necrosis factor-α (TNF-α) in serum were collected. NLRP3 inflammasome mRNA and Caspase-1 mRNA in peripheral blood mononuclear cells were detected by enzyme-linked immunosorbent assay(ELISA) and real-time quantitative polymerase chain reaction (qRT-PCR) respectively. Data were analyzed with t test, chi-square test or Fisher exact probability method, multivariate logistic regression and pearson correlation.

Results

There were no statistically significant differences in gender, age, hospitalization time, burns area, burns depth and IL-6 between the two groups (with P values above 0.05). The expression levels of IL-1β, IL-18, NLRP3 inflammasome mRNA and Caspase-1 mRNA in good prognosis group were (37.28±6.54) pg/mL, (38.26±8.79) pg/mL, 1.75±0.35 and 1.15±0.27, while those in poor prognosis group were (49.46±8.87) pg/mL, (76.83±10.58) pg/mL, 2.23±0.41 and 1.94±0.36. The differences between the two groups were statistically significant (t=5.11, 13.10, 4.17, 8.13; with P values below 0.05). Multivariate logistic regression analysis showed that the levels of IL-1β, IL-18, NLRP3 inflammasome mRNA and Caspase-1 mRNA were independent risk factors for poor prognosis of burn inhalation injury (β= 1.56, 0.87 1.05, 1.11; with P values below 0.05). Pearson correlation analysis showed that the levels of IL-1β, IL-18, NLRP3 inflammasome mRNA and Caspase-1 mRNA were positively correlated with the poor prognosis of burn inhalation injury (r= 0.42, 0.39, 0.52, 0.56; with P values below 0.05).

Conclusion

Abnormal elevated levels of IL-1β, IL-18, NLRP3 inflamesome, and Caspase-1 can be used as indicators of poor prognosis in patients with burn inhalation injury, and can guide clinical early treatment according to these indicators, which can help reduce the mortality of patients with burn combined with inhalation injury.

Key words: Burns, inhalation, Prognosis, Nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome, Inflammatory factor, Cysteine aspartic acid protease-1

Chao Sun, Huaxing Huang, Xian Deng. Correlation analysis of nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome, inflammatory factors, cysteine aspartic acid protease-1 and prognosis of burn combined with inhalation injury[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2020, 15(06): 428-433.

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References 22

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项目	上游引物	下游引物
NLRP3炎性小体mRNA	5′-CAT GAG TGC TGC TTC GAC AT-3′	5′-GCT TCA GTC CCA CAC AGA-3′
Caspase-1mRNA	5′-ATC GCT T TC TGC TCTTCC AC-3′	5′-TCC TCC ACA TCACAG GAA CA-3′

项目	上游引物	下游引物
NLRP3炎性小体mRNA	5′-CAT GAG TGC TGC TTC GAC AT-3′	5′-GCT TCA GTC CCA CAC AGA-3′
Caspase-1mRNA	5′-ATC GCT T TC TGC TCTTCC AC-3′	5′-TCC TCC ACA TCACAG GAA CA-3′

组别	例数	性别(例)		年龄(岁，±s)	烧伤后入院时间(h, ±s)	烧伤面积(%TBSA, ±s)
组别	例数	男	女	年龄(岁，±s)	烧伤后入院时间(h, ±s)	烧伤面积(%TBSA, ±s)
预后良好组	19	11	8	32.74±7.51	5.54±1.25	58.75±16.32
预后不良组	28	20	8	35.23±10.27	6.02±1.17	67.49±21.56
χ²值
t值		0.92		0.90	1.34	1.50
P值		0.34		0.37	0.19	0.14
组别	例数	烧伤深度(例)			NLRP3炎性小体mRNA(±s)	Caspase-1 mRNA(±s)
组别	例数	深Ⅱ度	Ⅲ度	Ⅳ度	NLRP3炎性小体mRNA(±s)	Caspase-1 mRNA(±s)
预后良好组	19	6	10	3	1.75±0.35	1.15±0.27
预后不良组	28	6	17	5	2.23±0.41	1.94±0.36
χ²值		0.61
t值					4.17	8.13
P值		0.74			<0.05	<0.05
组别	例数	炎性因子水平(pg/mL, ±s)
组别	例数	IL-1β	IL-6	IL-8	IL-18	TNF-α
预后良好组	19	37.28±6.54	55.39±12.73	376.46±38.61	38.26±8.79	7.85±1.33
预后不良组	28	49.46±8.87	60.72±14.38	402.80±45.68	76.83±10.58	8.14±1.05
t值		5.11	1.31	2.06	13.10	0.83
P值		<0.05	0.20	<0.05	<0.05	0.41

组别	例数	性别(例)		年龄(岁，±s)	烧伤后入院时间(h, ±s)	烧伤面积(%TBSA, ±s)
组别	例数	男	女	年龄(岁，±s)	烧伤后入院时间(h, ±s)	烧伤面积(%TBSA, ±s)
预后良好组	19	11	8	32.74±7.51	5.54±1.25	58.75±16.32
预后不良组	28	20	8	35.23±10.27	6.02±1.17	67.49±21.56
χ²值
t值		0.92		0.90	1.34	1.50
P值		0.34		0.37	0.19	0.14
组别	例数	烧伤深度(例)			NLRP3炎性小体mRNA(±s)	Caspase-1 mRNA(±s)
组别	例数	深Ⅱ度	Ⅲ度	Ⅳ度	NLRP3炎性小体mRNA(±s)	Caspase-1 mRNA(±s)
预后良好组	19	6	10	3	1.75±0.35	1.15±0.27
预后不良组	28	6	17	5	2.23±0.41	1.94±0.36
χ²值		0.61
t值					4.17	8.13
P值		0.74			<0.05	<0.05
组别	例数	炎性因子水平(pg/mL, ±s)
组别	例数	IL-1β	IL-6	IL-8	IL-18	TNF-α
预后良好组	19	37.28±6.54	55.39±12.73	376.46±38.61	38.26±8.79	7.85±1.33
预后不良组	28	49.46±8.87	60.72±14.38	402.80±45.68	76.83±10.58	8.14±1.05
t值		5.11	1.31	2.06	13.10	0.83
P值		<0.05	0.20	<0.05	<0.05	0.41

变量	β值	Wald值	P值	OR	95%CI
IL-1β	1.56	2.35	<0.05	4.75	1.168～8.735
IL-8	0.58	0.55	0.30	1.78	0.602～5.267
IL-18	0.87	4.51	0.01	2.37	1.592～14.312
NLRP3炎性小体mRNA	1.05	3.72	<0.05	2.86	1.099～10.261
Caspase-1 mRNA	1.11	2.94	<0.05	3.02	1.577～10.379

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