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中华损伤与修复杂志(电子版) ›› 2021, Vol. 16 ›› Issue (04) : 358 -361. doi: 10.3877/cma.j.issn.1673-9450.2021.04.014

综述

纤维环来源间充质干细胞的特点及提纯筛选法的研究进展
王曙光1, 张文捷2, 冯新民3, 南利平3, 王峰3, 朱磊3, 蔡同川3, 陈东3, 张亮3,()   
  1. 1. 116044 大连医科大学研究生院
    2. 530001 南宁,广西中医药大学附属国际壮医医院骨科
    3. 225001 扬州大学临床医学院
  • 收稿日期:2021-06-11 出版日期:2021-08-05
  • 通信作者: 张亮
  • 基金资助:
    国家自然科学基金面上项目(81972136); 广西省自然科学基金面上项目(2018JJA14775); 江苏省青年医学重点人才项目(QNRC2016342); 江苏省妇幼健康科研重点资助项目(F201801); 江苏省高层次卫生人才"六个一工程"拔尖人才科研项目(LGY2019035)

Characteristics of annulus fibrosus-derived mesenchymal stem cells and research progress of its purification and screening methods

Shuguang Wang1, Wenjie Zhang2, Xinmin Feng3, Liping Nan3, Feng Wang3, Lei Zhu3, Tongchuan Cai3, Dong Chen3, Liang Zhang3,()   

  1. 1. Graduate School, Daliang Medical University, Dalian 116044, China
    2. Department of Orthopedics, The Affiliated International Zhuang Yi Hospital of Guangxi Chinese Medicine University, Nanning 530001, China
    3. Clinical Medical College of Yangzhou University, Yangzhou 225001, China
  • Received:2021-06-11 Published:2021-08-05
  • Corresponding author: Liang Zhang
引用本文:

王曙光, 张文捷, 冯新民, 南利平, 王峰, 朱磊, 蔡同川, 陈东, 张亮. 纤维环来源间充质干细胞的特点及提纯筛选法的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2021, 16(04): 358-361.

Shuguang Wang, Wenjie Zhang, Xinmin Feng, Liping Nan, Feng Wang, Lei Zhu, Tongchuan Cai, Dong Chen, Liang Zhang. Characteristics of annulus fibrosus-derived mesenchymal stem cells and research progress of its purification and screening methods[J/OL]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(04): 358-361.

纤维环是椎间盘结构的重要组成部分,在椎间盘正常解剖结构及维持脊柱生物力学稳定性中发挥重要作用。纤维环细胞的损伤与凋亡是引起椎间盘退变的主要原因之一。现今生物学修复纤维环的主要方法有基因工程、细胞移植及组织工程等。已有研究表明,可以从纤维环组织中提取获得纤维环来源间充质干细胞(AFMSC),但因取材困难,退变纤维环提取的干细胞活力较差等问题,一定程度上限制了其应用。本文就AFMSC增殖特点、分化特点、分离提纯方法的研究进展作一综述。

Annulus fibrosus is an important part of intervertebral disc, which plays a vital role in maintaining biomechanical stability and forming normal anatomical structure of intervertebral disc. Annulus fibrosus cell injury and apoptosis are the main causes of intervertebral disc degeneration. At present, the methods to repair annulus fibrosus mainly include genetic engineering, cell transplantation and tissue engineering. Previous studies have shown that annulus fibrosus-derived mesenchymal stem cells (AFMSC) can be isolated from annulus fibrosus tissue. However, the utilization of the stem cells isolated from the degenerated annulus fibrosus is restricted due to the difficulty in obtaining the annulus fibrosus specimen and the poor cell viability. In this paper, the proliferation characteristics, differentiation characteristics, separation and purification methods of AFMSC and the research progress in separation and purification are reviewed.

[1]
Urits I, Viswanath O, Galasso AC, et al. Platelet-Rich Plasma for the Treatment of Low Back Pain: a Comprehensive Review[J]. Curr Pain Headache Rep, 2019, 23(7): 52.
[2]
Margarit C, Roca R, Inda MD, et al. Genetic Contribution in Low Back Pain: A Prospective Genetic Association Study[J]. Pain Pract, 2019, 19(8): 836-847.
[3]
Galliker G, Scherer DE, Trippolini MA, et al. Low Back Pain in the Emergency Department: Prevalence of Serious Spinal Pathologies and Diagnostic Accuracy of Red Flags[J]. Am J Med, 2020, 133(1): 60-72. e14.
[4]
Pavelka K, Jarosova H, Sleglova O, et al. Chronic Low Back Pain: Current Pharmacotherapeutic Therapies and a New Biological Approach[J]. Curr Med Chem, 2019, 26(6): 1019-1026.
[5]
Berg-Johansen B, Fields AJ, Liebenberg EC, et al. Structure-function relationships at the human spinal disc-vertebra interface[J]. J Orthop Res, 2018, 36(1): 192-201.
[6]
Zhou Q, Zhang JH, Yuan S, et al. A New Insight of Kartogenin Induced the Mesenchymal Stem Cells (MSCs) Selectively Differentiate into Chondrocytes by Activating the Bone Morphogenetic Protein 7 (BMP-7)/Smad5 Pathway[J]. Med Sci Monit, 2019, 25: 4960-4967.
[7]
Shukla S, Mittal SK, Foulsham W, et al. Therapeutic efficacy of different routes of mesenchymal stem cell administration in corneal injury[J]. Ocul Surf, 2019, 17(4): 729-736.
[8]
Richardson SM, Curran JM, Chen R, et al. The differentiation of bone marrow mesenchymal stem cells into chondrocyte-like cells on poly-L-lactic acid (PLLA) scaffolds[J]. Biomaterials, 2006, 27(22): 4069-4078.
[9]
王海. 来源于人退变椎间盘的三种干细胞与BM-MSCs生物学特性及组织工程应用的比较研究[D]. 重庆:第三军医大学,2013.
[10]
Feng G, Yang X, Shang H, et al. Multipotential differentiation of human anulus fibrosus cells: an in vitro study[J]. J Bone Joint Surg Am, 2010, 92(3): 675-685.
[11]
Yoshimura H, Muneta T, Nimura A, et al. Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle[J]. Cell Tissue Res, 2007, 327(3): 449-462.
[12]
Liang L, Li X, Li D, et al. The characteristics of stem cells in human degenerative intervertebral disc[J]. Medicine (Baltimore), 2017, 96(25): e7178.
[13]
Liu S, Liang H, Lee SM, et al. Isolation and identification of stem cells from degenerated human intervertebral discs and their migration characteristics[J]. Acta Biochim Biophys Sin (Shanghai), 2017, 49(2): 101-109.
[14]
Liu C, Guo Q, Li J, et al. Identification of rabbit annulus fibrosus-derived stem cells[J]. PloS One, 2014, 9(9): e108239.
[15]
Sang C, Cao X, Chen F, et al. Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus[J]. Stem Cells Int, 2016, 2016: 8283257.
[16]
吴鑫,胡琳,田明妹,等. 核干细胞因子的最新研究进展[J]. 四川生理科学杂志2013, 35(3): 115-118.
[17]
Frauchiger DA, Tekari A, May RD, et al. Fluorescence-Activated Cell Sorting Is More Potent to Fish Intervertebral Disk Progenitor Cells Than Magnetic and Beads-Based Methods[J]. Tissue Eng Part C Methods, 2019, 25(10): 571-580.
[18]
刘晨,赵泉来,王凌挺,等. 京尼平交联脱细胞纤维环基质/壳聚糖水凝胶与兔纤维环源干细胞的生物相容性[J]. 中国组织工程研究2016, 20(21): 3143-3149.
[19]
Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells[J]. Science, 1999, 284(5411): 143-147.
[20]
Beyer Nardi N, da Silva Meirelles L. Mesenchymal stem cells: isolation, in vitro expansion and characterization[J]. Handb Exp Pharmacol, 2006(174): 249-282.
[21]
Erwin WM, Las Heras F, Islam D, et al. The regenerative capacity of the notochordal cell: tissue constructs generated in vitro under hypoxic conditions[J]. J Neurosurg Spine, 2009, 10(6): 513-521.
[22]
金中行,徐宏光,张玙,等. 大鼠纤维环干细胞的分离与鉴定[J]. 皖南医学院学报2018, 37(4): 307-309, 313.
[23]
Wang H, Zhou Y, Chu TW, et al. Distinguishing characteristics of stem cells derived from different anatomical regions of human degenerated intervertebral discs[J]. Eur Spine J, 2016, 25(9): 2691-2704.
[24]
王晟昊,王奕彬,过倩萍,等. 脱细胞纤维环基质对兔纤维环源干细胞分化的影响[J]. 国际骨科学杂志2015, 36(3): 211-217.
[25]
Zhu C, Li J, Liu C, et al. Modulation of the gene expression of annulus fibrosus-derived stem cells using poly(ether carbonate urethane)urea scaffolds of tunable elasticity[J]. Acta Biomater, 2016, 29: 228-238.
[26]
Liu C, Zhu C, Li J, et al. The effect of the fibre orientation of electrospun scaffolds on the matrix production of rabbit annulus fibrosus-derived stem cells[J]. Bone Res, 2015, 3: 15012.
[27]
Chu G, Yuan Z, Zhu C, et al. Substrate stiffness- and topography-dependent differentiation of annulus fibrosus-derived stem cells is regulated by Yes-associated protein[J]. Acta Biomater, 2019, 92: 254-264.
[28]
黄锐,龚鑫. 电针对老年性痴呆大鼠前额叶皮质nNOS阳性神经元表达的影响[J]. 医学理论与实践2014, 27(8): 981-982, 1004.
[29]
马钢. miR-29b inhibitor调控骨髓间充质干细胞复合PLGA-HAp-ColⅠ双相支架修复骨软骨缺损的研究[D]. 重庆:重庆医科大学,2015.
[30]
范文帅,刘嘉,潘建锋,等. CD105+滑膜间充质干细胞成软骨能力[J]. 中华实验外科杂志2015, 32(1): 105-106.
[31]
唐勇,阳普山,吴剑宏,等. 人髓核间充质干细胞的分离提纯方式及生物学活性鉴定[J]. 中国脊柱脊髓杂志2015, 25(6): 533-540.
[32]
李淑娟. 磁性微粒及其在生物医药领域的应用[J]. 科技经济市场2008(10): 11-12.
[33]
张瑞,王俊芳,程力. 免疫磁珠分选兔前软骨干细胞及细胞研究平台的建立[J]. 中国矫形外科杂志2013, 21(13): 1352-1358.
[34]
王鹏程,游洪波,毛泽楷,等. CD105+滑膜间充质干细胞的磁珠分选及成软骨能力研究[J]. 中华实验外科杂志2017, 34(6): 921-923.
[35]
Neuhuber B, Swanger SA, Howard L, et al. Effects of plating density and culture time on bone marrow stromal cell characteristics[J]. Exp Hematol, 2008, 36(9): 1176-1185.
[36]
Benya PD, Shaffer JD. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels[J]. Cell, 1982, 30(1): 215-214.
[37]
袁超,王建,朱晓龙,等. 应力调节人椎间盘软骨终板干细胞成骨分化的研究[J]. 中国修复重建外科杂志2015, 29(3): 351-355.
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