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中华损伤与修复杂志(电子版) ›› 2021, Vol. 16 ›› Issue (04) : 358 -361. doi: 10.3877/cma.j.issn.1673-9450.2021.04.014

综述

纤维环来源间充质干细胞的特点及提纯筛选法的研究进展
王曙光1, 张文捷2, 冯新民3, 南利平3, 王峰3, 朱磊3, 蔡同川3, 陈东3, 张亮3,()   
  1. 1. 116044 大连医科大学研究生院
    2. 530001 南宁,广西中医药大学附属国际壮医医院骨科
    3. 225001 扬州大学临床医学院
  • 收稿日期:2021-06-11 出版日期:2021-08-05
  • 通信作者: 张亮
  • 基金资助:
    国家自然科学基金面上项目(81972136); 广西省自然科学基金面上项目(2018JJA14775); 江苏省青年医学重点人才项目(QNRC2016342); 江苏省妇幼健康科研重点资助项目(F201801); 江苏省高层次卫生人才"六个一工程"拔尖人才科研项目(LGY2019035)

Characteristics of annulus fibrosus-derived mesenchymal stem cells and research progress of its purification and screening methods

Shuguang Wang1, Wenjie Zhang2, Xinmin Feng3, Liping Nan3, Feng Wang3, Lei Zhu3, Tongchuan Cai3, Dong Chen3, Liang Zhang3,()   

  1. 1. Graduate School, Daliang Medical University, Dalian 116044, China
    2. Department of Orthopedics, The Affiliated International Zhuang Yi Hospital of Guangxi Chinese Medicine University, Nanning 530001, China
    3. Clinical Medical College of Yangzhou University, Yangzhou 225001, China
  • Received:2021-06-11 Published:2021-08-05
  • Corresponding author: Liang Zhang
引用本文:

王曙光, 张文捷, 冯新民, 南利平, 王峰, 朱磊, 蔡同川, 陈东, 张亮. 纤维环来源间充质干细胞的特点及提纯筛选法的研究进展[J]. 中华损伤与修复杂志(电子版), 2021, 16(04): 358-361.

Shuguang Wang, Wenjie Zhang, Xinmin Feng, Liping Nan, Feng Wang, Lei Zhu, Tongchuan Cai, Dong Chen, Liang Zhang. Characteristics of annulus fibrosus-derived mesenchymal stem cells and research progress of its purification and screening methods[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(04): 358-361.

纤维环是椎间盘结构的重要组成部分,在椎间盘正常解剖结构及维持脊柱生物力学稳定性中发挥重要作用。纤维环细胞的损伤与凋亡是引起椎间盘退变的主要原因之一。现今生物学修复纤维环的主要方法有基因工程、细胞移植及组织工程等。已有研究表明,可以从纤维环组织中提取获得纤维环来源间充质干细胞(AFMSC),但因取材困难,退变纤维环提取的干细胞活力较差等问题,一定程度上限制了其应用。本文就AFMSC增殖特点、分化特点、分离提纯方法的研究进展作一综述。

Annulus fibrosus is an important part of intervertebral disc, which plays a vital role in maintaining biomechanical stability and forming normal anatomical structure of intervertebral disc. Annulus fibrosus cell injury and apoptosis are the main causes of intervertebral disc degeneration. At present, the methods to repair annulus fibrosus mainly include genetic engineering, cell transplantation and tissue engineering. Previous studies have shown that annulus fibrosus-derived mesenchymal stem cells (AFMSC) can be isolated from annulus fibrosus tissue. However, the utilization of the stem cells isolated from the degenerated annulus fibrosus is restricted due to the difficulty in obtaining the annulus fibrosus specimen and the poor cell viability. In this paper, the proliferation characteristics, differentiation characteristics, separation and purification methods of AFMSC and the research progress in separation and purification are reviewed.

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