切换至 "中华医学电子期刊资源库"

中华损伤与修复杂志(电子版) ›› 2023, Vol. 18 ›› Issue (03) : 229 -234. doi: 10.3877/cma.j.issn.1673-9450.2023.03.009

论著

对人脐带间充质干细胞外泌体影响热损伤人皮肤成纤维细胞迁移的分析
黄瑞娟, 德奇, 巴特(), 周彪   
  1. 014010 包头,内蒙古包钢医院
    010110 呼和浩特,内蒙古医科大学第三临床医学院
  • 收稿日期:2022-07-01 出版日期:2023-06-01
  • 通信作者: 巴特
  • 基金资助:
    重大疾病防治科技行动计划(2018-ZX-01S-001); 内蒙古自治区自然科学基金项目(2020MS08022); 内蒙古自治区自然科学基金项目(2020MS08181); 内蒙古医科大学科技百万工程(联合)项目(YKD2016KJBW(LH)041); 内蒙古自治区科技创新引导项目(XCYD2020BT03)

Influence of human umbilical cord mesenchymal stem cell exosomes on migration of heat-damaged human skin fibroblasts

Ruijuan Huang, Qi De, Te Ba(), Biao Zhou   

  1. Laser Treatment Center, Inner Mongolia Baogang Hospital, Baotou 014010, China
    The Third Clinical Medical College of Inner Mongolia Medical University, Huhhot 010110, China
  • Received:2022-07-01 Published:2023-06-01
  • Corresponding author: Te Ba
引用本文:

黄瑞娟, 德奇, 巴特, 周彪. 对人脐带间充质干细胞外泌体影响热损伤人皮肤成纤维细胞迁移的分析[J/OL]. 中华损伤与修复杂志(电子版), 2023, 18(03): 229-234.

Ruijuan Huang, Qi De, Te Ba, Biao Zhou. Influence of human umbilical cord mesenchymal stem cell exosomes on migration of heat-damaged human skin fibroblasts[J/OL]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(03): 229-234.

目的

初步探讨人脐带间充质干细胞外泌体对热损伤人皮肤成纤维细胞迁移的影响。

方法

分离脐带间充质干细胞(hucMSCs)并进行形态学鉴定,流式细胞术检测细胞表面标志物。采用PEG沉淀法联合差速离心法获取人脐带间充质干细胞外泌体(hucMSC-Exos)并鉴定。分离培养出人真皮成纤维细胞,建立成纤维细胞热损伤模型。将热损伤成纤维细胞分为3组,分别加入不同剂量(0 μg、50 μg、100 μg)hucMSC-Exos溶液,依次进行细胞划痕、Transwell实验,观察hucMSC-Exos对热损伤成纤维细胞迁移的影响。

结果

24 h后,50 μg和100 μg hucMSC-Exos组热损伤成纤维细胞的划痕愈合率分别为42.86%±6.54%、55.42%±8.53%,均大于0 μg hucMSC-Exos组(23.21%±8.25%),差异有统计学意义(t=-7.111、-9.268,P<0.05)。24 h后,50 μg、100 μg hucMSC-Exos组热损伤成纤维细胞迁移个数分别为(31.47±4.31)、(39.53±3.11)个,相比于0 μg hucMSC-Exos组[(19.20±2.91)个]显著增多,组间比较差异有统计学意义(t=-10.213、-28.710,P<0.05)。

结论

人脐带间充质干细胞外泌体能够促进热损伤人皮肤成纤维细胞迁移。

Objective

To preliminarily investigate the effect of human umbilical cord mesenchymal stem cell exosomes on the migration of heat-damaged human skin fibroblasts.

Methods

Umbilical cord mesenchymal stem cells (hucMSCs) were isolated and morphologically identified surface markers by flow cytometry. Human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exos) were obtained and identified by PEG precipitation combined with differential centrifugation. Human dermal fibroblasts were isolated and cultured, and a model of fibroblast thermal injury was established. Heat-damaged fibroblasts were divided into three groups, different doses of hucMSC-Exos solution (0 μg, 50 μg, 100 μg) were added, and cell scratches and Transwell experiments were performed sequentially to observe the effect of hucMSC-Exos on the migration of heat-damaged fibroblasts.

Results

After 24 h, the scratch healing rates of heat-damaged fibroblasts in 50 μg and 100 μg hucMSC-Exos groups were 42.86%±6.54% and 55.42%±8.53%, respectively, which were higher than that in 0μg hucMSC-Exos group (23.21%±8.25%) with statistically differences (t=-7.111、-9.268, P<0.05). After 24 h, the migration numbers of heat-damaged fibroblasts in 50 μg and 100 μg hucMSC-Exos groups were 31.47±4.31 and 39.53±3.11, respectively, which were more than that in 0 μg hucMSC-Exos group (19.20±2.91), the differences were statistically significant (t=-10.213、-28.710, P<0.05).

Conclusion

Human umbilical cord mesenchymal stem cell exosomes can promote the migration of heat-damaged human skin fibroblasts.

图1 脐带间充质干细胞、外泌体、成纤维细胞培养及鉴定。A示脐带间充质干细胞流式细胞仪鉴定;B示脐带间充质干细胞传代培养(100×);C示外泌体透射透射电子显微镜观察;D示外泌体粒径分析;E示成纤维细胞传代培养(50×);F示成纤维细胞波形蛋白染色(100×)
图2 细胞热损伤模型建立及评估。A示细胞划痕实验(50×);B示Transwell实验(100×)
图3 hucMSC-Exos对热损伤成纤维细胞迁移的影响。A示细胞划痕实验(50×); B示Transwell实验(100×)
[1]
Wang C, Wang M, Xu T, et al. Engineering bioactive self-healing antibacterial exosomes hydrogel for promoting chronic diabetic wound healing and complete skin regeneration[J]. Theranostics, 2019, 9(1): 65-76.
[2]
An Y, Lin S, Tan X, et al. Exosomes from adipose-derived stem cells and application to skin wound healing[J]. Cell Proliferation, 2021, 54(3): e12993.
[3]
Shi A, Arrell D, Peterson TE, et al. Abstract 14765: exosome shuttled VEGFR promotes angiogenesis in peripheral vascular disease[J]. Circulation, 2020
[4]
Qi J, Liu Q, Reisdorf RL, et al. Characterization of a purified exosome product and its effects on canine flexor tenocyte biology[J]. J Orthop Res, 2020, 38(8): 1845-1855.
[5]
Shi A, Li J, Qiu X, et al. TGF-βloaded exosome enhances ischemic wound healing in vitro and in vivo[J]. Theranostics, 2021, 11(13): 6616-6631.
[6]
Woodley DT. Distinct fibroblasts in the papillary and reticular dermis: implications for wound healing[J]. Dermatol Clin, 2017, 35(1): 95-100.
[7]
DesJardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update[J]. Regenerative Medicine, 2018, 13(5): 491-495.
[8]
Li Z, Maitz P. Cell therapy for severe burn wound healing[J]. Burns & Trauma, 2018, 6: 13.
[9]
Pelizzo G, Avanzini MA, Mantelli M, et al. Granulation tissue-derived mesenchymal stromal cells: a potential application for burn wound healing in pediatric patients[J]. Journal of Stem Cells and Regenerative Medicine, 2018, 14(1): 53-58.
[10]
Matsuura Y, Noda K, Suzuki S, et al. Glucocorticoids suppress fibroblast apoptosis in an in vitro thermal injury model[J]. Burns, 2019, 45(1): 173-179.
[11]
Cao W, Feng Y. LncRNA XIST promotes extracellular matrix synthesis, proliferation and migration by targeting miR-29b-3p/COL1A1 in human skin fibroblasts after thermal injury[J]. Biological Research, 2019, 52(1): 52.
[12]
熊亮,胡寅,王立夫,等. miRNA-199a-5p在热损伤人皮肤成纤维细胞中的表达及其功能研究[J]. 南京医科大学学报(自然科学版), 2021, 41(8): 1160-1165.
[13]
李德全,黄晓元,杨兴华. 离体人皮肤成纤维细胞热损伤后转归的形态学观察[J]. 中国组织工程研究与临床康复2008, 12(11): 2056-2060.
[14]
左琛琛. PPARβ对成纤维细胞热损伤变性的保护作用及其机制研究[D]. 中南大学,2012.
[15]
朱雅楠,李维龙,郭晓宇,等. 过氧化物还原酶Ⅱ在热损伤诱导HaCaT细胞凋亡中的保护作用[J]. 黑龙江八一农垦大学学报2019, 31(6): 72-77.
[16]
叶田园,李志勇,李彦文,等. 地骨皮提取物对热损伤大鼠皮肤成纤维细胞的保护作用[J]. 时珍国医国药2015, 26(11): 2641-2643.
[17]
吕春柳. miRNAs在变性真皮及热损伤成纤维细胞/HUVECs中表达及功能的研究[D]. 中南大学,2013.
[18]
Greenhalgh DG. Management of Burns[J]. N Engl J Med, 2019, 380(24): 2349-2359.
[19]
Rahman MS, Islam R, Rana MM, et al. Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract[J]. BMC Complementary and Alternative Medicine, 2019, 19(1): 115.
[20]
Wang M, Xu X, Lei X, et al. Mesenchymal stem cell-based therapy for burn wound healing[J]. Burns Trauma, 2021, 9: b2.
[21]
Han C, Sun X, Liu L, et al. Exosomes and their therapeutic potentials of stem cells[J]. Stem Cells International, 2016, 2016: 1-11.
[22]
李超然,黄桂林,王帅. 间充质干细胞来源外泌体促进损伤组织修复与再生的应用与进展[J]. 中国组织工程研究2018, 22(1): 7.
[23]
王倩,肖文华. 肿瘤来源外泌体在机体免疫系统与肿瘤微环境中的作用及其临床应用前景[J]. 解放军医学院学报2017, 38(1): 4.
[1] 宋勤琴, 李双汝, 李林, 杜鹃, 刘继松. 间充质干细胞源性外泌体在改善病理性瘢痕中作用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 550-553.
[2] 刘昌玲, 张金丽, 张志, 李孝建, 汤文彬, 胡逸萍, 陈宾, 谢晓娜. 负载人脂肪干细胞外泌体的甲基丙烯酰化明胶水凝胶对人皮肤成纤维细胞增殖和迁移的影响[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 517-525.
[3] 雷子威, 凌萍, 沈纵, 魏晨如, 朱邦晖, 伍国胜, 孙瑜. 类器官肺损伤疾病模型构建及应用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 531-535.
[4] 关丁丁, 李伟, 孔维诗, 包郁露, 孙瑜. 负载干细胞的光交联蛋白基水凝胶在组织工程中应用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(05): 447-452.
[5] 傅红兴, 王植楷, 谢贵林, 蔡娟娟, 杨威, 严盛. 间充质干细胞促进胰岛移植效果的研究进展[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(06): 351-360.
[6] 王大伟, 陆雅斐, 皇甫少华, 陈玉婷, 陈澳, 江滨. 间充质干细胞通过调控免疫机制促进创面愈合的研究进展[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(06): 361-366.
[7] 刘文竹, 唐窈, 刘付臣. 诱导多潜能干细胞在神经肌肉疾病研究中的应用进展[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(06): 367-373.
[8] 袁园园, 岳乐淇, 张华兴, 武艳, 李全海. 间充质干细胞在呼吸系统疾病模型中肺组织分布及治疗机制的研究进展[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(06): 374-381.
[9] 赵旭鹏, 王集琛, 田硕, 李宏召, 李修彬, 张旭. EP300 通过上调FKBP10 促进膀胱肿瘤细胞迁移和侵袭[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(05): 264-274.
[10] 蔡艺丹, 方坚, 张志强, 陈莉, 张世安, 夏磊, 阮梅, 李东良. 经颈静脉肝内门体分流术对肝硬化门脉高压患者肠道菌群及肝功能的影响[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(05): 285-293.
[11] 陈惠燕, 吴瑶, 黄宗炫, 卜歆, 王庆惠, 纪辉涛, 陈银珍, 赵虎. 肾间质纤维化中胶原/DDR2 信号活化对肾成纤维细胞增殖和迁移功能影响的实验研究[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(05): 294-302.
[12] 王俊楠, 刘晔, 李若涵, 叶青松. 间充质干细胞调控肠脑轴治疗神经系统疾病的潜力[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(05): 313-319.
[13] 袁雨涵, 杨盛力. 体液和组织蛋白质组学分析在肝癌早期分子诊断中的研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 883-888.
[14] 王向前, 李清峰, 陈磊, 丘文丹, 姚志成, 李熠, 吴荣焕. 姜黄素抑制肝细胞癌索拉非尼耐药作用及其调控机制[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 729-735.
[15] 王国强, 张纲, 唐建坡, 张玉国, 杨永江. LINC00839 调节miR-17-5p/WEE1 轴对结直肠癌细胞增殖、凋亡和迁移的影响[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 491-499.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?