To investigate the effect of Xuebijing on vascular permeability and fluid demand in burn dogs.

Twenty four male Beagle dogs were divided into 3 groups according to the random number table method: lactated ringer′s solution resuscitation group (LR group), lactated Ringer′s solution resuscitation+ Xuebijing group (LR+ XBJ group) and control group (OC group), 8 dogs in each group. All the dogs were anesthetized and then the carotid aorta and venous catheters were placed and urinary catheters were inserted. All the dogs in the 3 groups were injected intravenously with propofol (2 mg/kg) for general anesthesia. The dogs in the OC group received only jugular arteriovenous catheterization without injury. In the LR group and LR+ XBJ group, after jugular arteriovenous catheterization, 50% of the total body surface area of the back was measured. After skin preparation, 3% naphtha was evenly smeared and ignited, and continued to burn for 30 s, resulting in 50% of the total body surface area of the burn. Twelve hours after the injury, buprenorphine (10 μg/kg) was given to relieve pain. In the LR group, the lactated Ringer′s solution was intravenously injected according to the Parkland formula (1% of the total body surface area was 4 mL/kg, and the first 8 h was entered as 1/2, the second 16 h as the other entered); in the LR+ XBJ group, based on the lactated Ringer′s solution (the input amount and time were the same as those in the LR group), Xuebijing injection was given at a dose of 2 mL/kg immediately after the injury and 4 h after the injury. In the OC group, only the same amount of lactated Ringer′s solution as the LR group and the LR+ XBJ group was input. The hemodynamic indicators [mean arterial pressure (MAP), cardiac output index (CI), extravascular lung water index (ELWI) and pulmonary vascular permeability index (PVPI)], plasma volume, hematocrit, urine accumulation and cumulative net input, moisture content of various organ tissues (heart, lung, kidney, small intestine), vascular permeability, neutrophil infiltration number, and myeloperoxidase (MPO) and neutrophil elastase(NE) at immediately after injury, 1, 2, 4, 6, 8, and 24 h after injury were detected. Data were compared with one-way analysis of variance, t test, and χ² test.

Immediately after injury, there were no statistically significant differences in MAP, CI, ELWI, and PVPI between the 3 groups (with P values above 0.05); 1, 2, 4, 6, 8, and 24 h after injury, the MAP, CI, ELWI, and PVPI in the LR group and LR+ XBJ group were significantly lower than those in the OC group, and the differences between the 3 groups were statistically significant (with P values below 0.05). At 1, 2, 4, 6, 8 and 24 h after injury, there were no statistically significant difference in MAP and CI between the LR group and the LR+ XBJ group (with P values above 0.05); there were no statistically significant differences in ELWI and PVPI between the LR group and the LR+ XBJ group at 1, 2 and 4 h after injury (with P values above 0.05), but at 6, 8, and 24 h after injury, the ELWI and PVPI of the two groups were statistically significant (with P values below 0.05). Immediately after injury, there were no statistically significant differences in plasma volume and hematocrit between the three groups (with P values above 0.05); at 1, 2, 4, 6, 8 and 24 h after injury, the plasma volume and hematocrit in the LR group and the LR+ XBJ group were significantly lower than those in the OC group, the differences of the 3 groups were statistically significant (with P values below 0.05); at 1, 2, 6, 8, and 24 h after injury, there were no statistically significant difference in plasma volume between the LR group and the LR+ XBJ group (with P values above 0.05); compared with the LR+ XBJ group, the plasma volume of the LR group at 4 h after injury was statistically significant (P<0.05); at 1, 6, 8, and 24 h after injury, the hematocrit of the two groups were no statistically significant (with P values above 0.05), and the hematocrit of the two groups at 2 and 4 h after injury were statistically significant (with P values below 0.05). Immediately after injury, 1, 2, 4, 6, 8, and 24 h after injury, there was no statistically significant difference in urine output between LR group and LR+ XBJ group (with P values above 0.05). Immediately after injury, 1, 2 h after injury, the cumulative net input in the LR group and LR+ XBJ group were no statistically significant(with P values above 0.05), but at 4, 6, 8 and 24 h after injury, the cumulative net input in the two groups were statistically significant (with P values below 0.05). At 24 h after injury, the water content, vascular permeability, and neutrophil infiltration in the heart, lung, kidney, and small intestine of the LR group and LR+ XBJ groups were significantly higher than those in the OC group, the differences of the three group was statistically significant (with P values below 0.05); at 24 h after injury, the water content and vascular permeability in the heart and kidney of the LR group were no statistically significantly different from those in the LR+ XBJ group (with P values above 0.05), and in the lung and small intestine were statistically significant (with P values below 0.05). At 24 h after injury, there was no significant difference in the number of neutrophil infiltration in the kidney tissue of the LR group and LR+ XBJ group (P>0.05), the number of neutrophil infiltration in the heart, lung, and small intestine in the 2 groups were statistically significant (with P values below 0.05). At 24 h after injury, the contents of MPO and NE in the heart, lung, kidney, and small intestine of the LR group and LR+ XBJ group were significantly higher than those in the OC group, the differences between the 3 groups were statistically significant (with P values below 0.05); 24 h after injury, the MPO content in heart and kidney tissues of LR group was not significantly difference from that of LR+ XBJ group (P=0.05, P>0.05); compared with the LR+ XBJ group, the MPO content in the lung and small intestine in the LR group were statistically significant (with P values below 0.05). At 24 h after injury, there was no statistically significant difference in NE content in kidney tissue between LR group and LR+ XBJ group (P>0.05). The differences of NE content in heart, lung, and small intestine of the two groups were statistical significance (with P values below 0.05).

Xuebijing injection can inhibit the increase of vascular permeability and tissue edema caused by protease, and significantly reduce the resuscitation fluid demand of burn dogs.