To preliminarily investigate the effect of human umbilical cord mesenchymal stem cell exosomes on the migration of heat-damaged human skin fibroblasts.

Umbilical cord mesenchymal stem cells (hucMSCs) were isolated and morphologically identified surface markers by flow cytometry. Human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exos) were obtained and identified by PEG precipitation combined with differential centrifugation. Human dermal fibroblasts were isolated and cultured, and a model of fibroblast thermal injury was established. Heat-damaged fibroblasts were divided into three groups, different doses of hucMSC-Exos solution (0 μg, 50 μg, 100 μg) were added, and cell scratches and Transwell experiments were performed sequentially to observe the effect of hucMSC-Exos on the migration of heat-damaged fibroblasts.

After 24 h, the scratch healing rates of heat-damaged fibroblasts in 50 μg and 100 μg hucMSC-Exos groups were 42.86%±6.54% and 55.42%±8.53%, respectively, which were higher than that in 0μg hucMSC-Exos group (23.21%±8.25%) with statistically differences (t=-7.111、-9.268, P<0.05). After 24 h, the migration numbers of heat-damaged fibroblasts in 50 μg and 100 μg hucMSC-Exos groups were 31.47±4.31 and 39.53±3.11, respectively, which were more than that in 0 μg hucMSC-Exos group (19.20±2.91), the differences were statistically significant (t=-10.213、-28.710, P<0.05).

Human umbilical cord mesenchymal stem cell exosomes can promote the migration of heat-damaged human skin fibroblasts.