Effect of negative pressure wound therapy on proliferation of epidermal stem cell in elderly diabetic ulcer wounds

doi:10.3877/cma.j.issn.1673-9450.2017.06.006

Abstract

Abstract:

Objective

To study on the changes of epidermal stem cells proliferation in the treatment of elderly diabetic ulcer wound by negative-pressure wound therapy (NPWT).

Methods

Elderly patients with diabetes mellitus complicated with chronic refractory wounds were selected from First Affiliated Hospital, Sun Yat-sen University and Shenzhen Longhua District Central Hospital from January 2011 to December 2015. NPWT was used to treat these wounds. Immunohistochemical method was used to detect the changes of integrin beta 1 in basal cells of wound healing process(respectively before treatment, 2 weeks after treatment, 4 weeks after treatment). The paired-samples t test was used.

Results

No integrin beta 1 positive cells were found in the basal cells of the limbus wound before the NPWT, the integrin beta 1 positive cells appeared at the periphery of the wound at 2 weeks after NPWT, the positive unit was 1.74±0.07. At 4 weeks after received NPWT, the positive unit (expression of integrin beta 1 positive cells) was 29.07±0.65. The expression of epidermal stem cells (integrin beta 1) at the 2 and 4 weeks after received NPWT was significantly different from that before received NPWT (t=75.29, 140.31, with P values below 0.05). There was significant difference in the expression of epidermal stem cells (integrin beta 1) at the 4 weeks after received NPWT and 2 weeks after received NPWT (t=134.25, P<0.05).

Conclusion

NPWT promotes the proliferation of epidermal stem cells in elderly diabetic ulcer wounds and promotes epithelial wound healing.

Key words: Negative-pressure wound therapy, Diabetes mellitus, Ulcer, Aged, Cell proliferation, Epidermal stem cells

Po Liu, Changneng Ke, Bin Shu, Weishan Li, Zhiyuan Tong, Xiangmei Wang, Zhongqiang Zhang, Songyun Zou. Effect of negative pressure wound therapy on proliferation of epidermal stem cell in elderly diabetic ulcer wounds[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2017, 12(06): 432-436.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhssyxfzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1673-9450.2017.06.006

https://zhssyxfzz.cma-cmc.com.cn/EN/Y2017/V12/I06/432

Figures/Tables 2

References 20

[1]	Dumville JC, Hinchliffe RJ, Cullum N, et al. Negative pressure wound therapy for treating foot wounds in people with diabetes mellitus[J]. Cochrane Database Syst Rev, 2013, 17(10):CD010318.
[2]	Suess JJ, Kim PJ, Steinberg JS. Negative pressure wound therapy: evidence-based treatment for complex diabetic foot wounds[J]. Curr Diab Rep, 2006, 6(6):446-450.
[3]	Ramanujam CL, Stapleton JJ, Zgonis T. Negative-pressure wound therapy in the management of diabetic Charcot foot and ankle wounds[J]. Diabet Foot Ankle, 2013, 4:20878.
[4]	Seidel D, Mathes T, Lefering R, et al. Negative pressure wound therapy versus standard wound care in chronic diabetic foot wounds: study protocol for a randomized controlled trial[J]. Trials, 2014, 15:334.
[5]	刘坡，祁少海，谢举临，等. 老年人慢性难愈合创面的治疗[J/CD]. 中华损伤与修复杂志(电子版), 2012, 7(4):396-398.
[6]	Dalla PL. Diabetic foot wounds: the value of negative pressure wound therapy with instillation[J]. Int Wound J, 2013, 10 Suppl 1:25-31.
[7]	Hasan MY, Teo R, Nather A. Negative-pressure wound therapy for management of diabetic foot wounds: a review of the mechanism of action, clinical applications, and recent developments[J]. Diabet Foot Ankle, 2015, 6:27618.
[8]	Yang SL, Zhu LY, Han R, et al. Effect of Negative Pressure Wound Therapy Cellular Fibronectin and Transforming Growth Factor-β1 Expression in Diabetic Foot Wounds[J]. Foot Ankle Int, 2017, 38(8):893-900.
[9]	Yang SL, Han R, Liu Y, et al. Negative pressure wound therapy is associated with up-regulation of bFGF and ERK1/2 in human diabetic foot wounds[J]. Wound Repair Regen, 2014, 22(4):548-554.
[10]	杨少玲，孙蕾蕾，胡丽叶，等. 负压伤口治疗对糖尿病足创面肉芽组织血小板源生长因子表达的影响[J/CD]. 中华损伤与修复杂志(电子版), 2016, 11(2):107-112.
[11]	Ma Z, Shou K, Li Z, et al. Negative pressure wound therapy promotes vessel destabilization and maturation at various stages of wound healing and thus influences wound prognosis[J]. Exp Ther Med, 2016, 11(4):1307-1317.
[12]	申洪. 免疫组织化学染色定量方法研究(Ⅲ)[J]. 中国组织化学与细胞化学杂志，1995, 4(1):89-92.
[13]	Motley TA, Gilligan AM, Lange DL, et al. Cost-effectiveness of clostridial collagenase ointment on wound closure in patients with diabetic foot ulcers: economic analysis of results from a multicenter, randomized, open-label trial[J]. J Foot Ankle Res, 2015, 8:7.
[14]	Aumiller WD, Dollahite HA. Pathogenesis and management of diabetic foot ulcers[J]. JAAPA, 2015, 28(5):28-34.
[15]	关小宏，李宝军，肖黎，等. 糖尿病足流行病学及糖尿病足截肢(趾)的临床情况分析[J/CD]. 中华损伤与修复杂志(电子版), 2012, 7(4):406-408.
[16]	陈波，贲道锋，夏照帆. 负压创面治疗技术的研究应用进展[J/CD]. 中华损伤与修复杂志(电子版), 2014, 9(2):198-202.
[17]	Shankaran V, Brooks M, Mostow E. Advanced therapies for chronic wounds: NPWT, engineered skin, growth factors, extracellular matrices[J]. Dermatol Ther, 2013, 26(3):215-221.
[18]	Ernst N, Yay A, Bíró T, et al. β1 integrin signaling maintains human epithelial progenitor cell survival in situ and controls proliferation, apoptosis and migration of their progeny[J]. PLoS One, 2013, 8(12):e84356.
[19]	Giangreco A, Goldie SJ, Failla V, et al. Human skin aging is associated with reduced expression of the stem cell markers beta1 integrin and MCSP[J]. J Invest Dermatol, 2010, 130(2):604-608.
[20]	Li JF, Duan HF, Wu CT, et al. HGF accelerates wound healing by promoting the dedifferentiation of epidermal cells through β1-integrin/ILK pathway[J]. Biomed Res Int, 2013, 2013:470418.

Please choose a citation manager

Content to export