Confusion, challenge and hope on diagnosis of tuberculous wound

doi:10.3877/cma.j.issn.1673-9450.2020.06.001

Abstract

Abstract:

The clinical diagnosis of tuberculous wound is still a difficult problem for doctors to implement accurate treatment. Although some traditional diagnostic methods like mycobacterium tuberculosis isolation and culture, acid-fast staining, histopheological examination, tuberculin test, chromatography, serum immunology and molecular biology diagnosis are all in use, there are generally defects such as complex operation process, long cycle and low positive rate. The challenge in clinical diagnosis of tuberculous wound is to find a rapid, convenient, concise and highly positive method. New molecular biology technology real-time fluorescence quantitative polymerase chain reaction (PCR) probe fusion curve method has shown its unique advantages in the diagnosis and drug resistance analysis of the paraffin-embedded tissue of tuberculous wound. It is short in time, easy to operate, and can identify multiple mycobacterium tuberculosis and non-mycobacterium tuberculosis at the same time, with high specificity and sensitivity. It brings new hope for the difficult problem of clinical diagnosis of tuberculous wound.

Key words: Mycobacterium tuberculosis, Diagnosis, Tuberculous wound, Challenges and hopes, Polymerase chain reaction melting curve analysis

Chiyu Jia, Shuting Mao. Confusion, challenge and hope on diagnosis of tuberculous wound[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2020, 15(06): 423-427.

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