切换至 "中华医学电子期刊资源库"
高级检索
中华损伤与修复杂志(电子版)

中华损伤与修复杂志(电子版) ›› 2016, Vol. 11 ›› Issue (02) : 90 -95. doi: 10.3877/cma.j.issn.1673-9450.2016.02.003

所属专题： 文献；

论著

丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响
唐富波1, 张文华2, 李雨梦2, 李静远2, 刘锐3, 胡森2, 白晓东4,†()   
  1. 1. 100039　北京，武警总医院烧伤整形科；300309　天津，武警后勤学院
    2. 100048　北京，解放军总医院第一附属医院全军创伤修复与组织再生重点实验室暨皮肤损伤修复与组织再生北京市重点实验室
    3. 150040　哈尔滨市第五医院烧伤整形科
    4. 100039　北京，武警总医院烧伤整形科
  • 收稿日期:2015-12-10 出版日期:2016-04-01
  • 通信作者: 白晓东
  • 基金资助:
    国家自然科学基金(81471872); 国家发改委卫星示范专项(发改办高技（2013）2140号)

Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury

Fubo Tang1, Wenhua Zhang2, Yumeng Li2, Jingyuan Li2, Rui Liu3, Sen Hu2, Xiaodong Bai4,†()   

  1. 1. Department of Burn and Plastic Surgery, the General Hospital of Armed Police Forces, Beijing 100039, China; Logistics University of Chinese People′s Armed Police Forces, Tianjin 300309, China
    2. Laboratory for Shock and Multiple Organ Dysfunction, Key Research Laboratory of Tissue Repair and Regeneration of People′s Liberation Army, and Beijing Key Research Laboratory of Skin Injury and Repair Regeneration, the First Hospital Affiliated to General Hospital of People′s Liberation Army, Beijing 100048, China
    3. Department of Burn and Plastic Surgery, the Fifth Hospital of Harbin, Harbin 150040, China
    4. Department of Burn and Plastic Surgery, the General Hospital of Armed Police Forces, Beijing 100039, China
  • Received:2015-12-10 Published:2016-04-01
  • Corresponding author: Xiaodong Bai
  • About author:
    Corresponding author: Bai Xiaodong, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

唐富波, 张文华, 李雨梦, 李静远, 刘锐, 胡森, 白晓东. 丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响[J/OL]. 中华损伤与修复杂志(电子版), 2016, 11(02): 90-95.

Fubo Tang, Wenhua Zhang, Yumeng Li, Jingyuan Li, Rui Liu, Sen Hu, Xiaodong Bai. Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury[J/OL]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2016, 11(02): 90-95.

目的

研究丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响。

方法

雄性SD大鼠48只，体重(250±20)g，随机数字表法分为假伤组、烧伤组和丁酸钠组，每组16只。丁酸钠组与烧伤组采用沸水烫大鼠背部15 s、腹部8 s，造成50%TBSAⅢ度烧伤，伤后分别立即腹腔注射丁酸钠(400 mg/kg)与等体积0.9%氯化钠溶液。假伤组37℃温水浸泡后腹腔注射等体积0.9%氯化钠溶液。伤后3 h和6 h采用激光多普勒血流仪测定肝、肾、小肠黏膜血流量；腹主动脉取血检测血清谷丙转氨酶(ALT)、肌酐(Cr)、肌酸激酶同工酶(CK- MB)和二胺氧化酶(DAO)；处死大鼠取脏器组织采用干湿重法检测心、肺、肝、肾和小肠含水率。

结果

丁酸钠组伤后3 h肾和小肠黏膜血流量[(93.56±11.56)、(84.65±12.64)BPU]分别高于烧伤组[(80.71±10.53)、(59.64±11.82)BPU]，差异均有统计学意义(t＝2.324、4.087，P值均小于0.05)；心、肺、小肠含水率[(72.35±1.93)%、(69.56±1.83)%、(63.75±2.58)%]以及CK- MB水平(2794.56±291.54)U/L分别低于烧伤组(74.45±1.62)%、(73.56±1.69)%、(72.54±2.93)%、(3676.32±259.65)U/L，差异均有统计学意义(t＝2.357、4.541、6.368、6.388，P值均小于0.05)。丁酸钠组伤后6 h肝和小肠黏膜血流量[(65.36±11.79)、(62.65±12.56)BPU]分别高于烧伤组[(51.72±10.54)、(31.56±12.72)BPU]，差异均有统计学意义(t＝2.439、4.919，P值均小于0.05)；肺、肝、肾和小肠含水率[(73.72±2.05)%、(78.41±1.84)%、(75.64±2.63)%、(70.53±3.13)%]分别低于烧伤组[(80.62±2.16)%、(82.62±1.93)%、(80.32±3.05)%、(81.53±2.79)%]，差异均有统计学意义(t＝6.553、4.465、3.286、7.420，P值均小于0.05)；丁酸钠组血浆ALT(96.36±6.56)U/L、Cr(79.35±4.16)μmol/L、DAO(78.54±5.23)U/L、CK- MB(3712.64±309.45)U/L分别低于烧伤组[(113.54±7.41)U/L、(90.34±5.37)μmol/L、(92.34±5.34)U/L、(5264.46±351.62)U/L]，差异均有统计学意义(t＝4.910、4.576、5.222、9.370，P值均小于0.05)。

结论

丁酸钠能够增加特重度烧伤大鼠腹腔脏器血流量，减轻组织水肿，改善脏器功能，对特重度烧伤引起的重要脏器损害具有一定的保护作用。

关键词: 组蛋白脱乙酰基酶抑制剂, 烧伤, 水肿, 丁酸钠, 脏器功能, 血流量
Objective

To investigate the effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury.

Methods

Forty-eight male SD rats whose average weight were (250±20) grams were randomly divided into sham group, burn injury group and sodium butyrate group (n=16). Rats of burn injury group and sodium butyrate group were subjected to extraordinarily severe burn injury by scalding the rats′ back 15 s and abdomen 8 s with boiling water. And then sodium butyrate solution (400 mg/kg) or same volume of 0.9% sodium chloride solution was intraperitoneally injected, respectively. While the same volume of 0.9% sodium chloride solution was intraperitoneally injected into rats of sham group after soaking in 37 ℃ warm water. At 3 and 6 hours after injury, the blood flow of liver, kidney and small intestine were measured by laser doppler flowmetry, and blood samples were drawn from abdominal aorta for measurement of parameters of organ function and the activity of diamine oxidase. The tissues samples of kidney, liver, heart, lung and small intestine were harvested for the measurement of tissue water content.

Results

Compared with burn injury group at 3 hours after injury, the blood flow of kidney and small intestine in sodium butyrate group were (93.56±11.56) BPU and (84.65±12.64)BPU, significantly higher than those in burn injury group[(80.71±10.53) BPU, (59.64±11.82)BPU], the difference was statistically significant(t=2.324, 4.087, all P values were less than 0.05). The water content of heart, lung and small intestine in sodium butyrate group were (72.35±1.93)%, (69.56±1.83)%, (63.75±2.58)%, and the level of creatine kinase MB isoenzyme was(2794.56±291.54)U/L, significantly lower than those in burn injury group[(74.45±1.62)%, (73.56±1.69)%, (72.54±2.93)%] and (3676.32±259.65)U/L, the difference was statistically significant(t=2.357, 4.541, 6.368, 6.388, all P values were less than 0.05). Compared with burn injury group at 6 hours after injury, the blood flow of liver and small intestine in sodium butyrate group were (65.36±11.79) BPU, (62.65±12.56)BPU, significantly higher than those in burn injury group[(51.72±10.54) BPU, (31.56±12.72)BPU], the difference was statistically significant(t=2.439, 4.919, all P values were less than 0.05). The water content of lung, liver, kidney and small intestine in sodium butyrate group were (73.72±2.05)%, (78.41±1.84)%, (75.64±2.63)%, (70.53±3.13)%, significantly lower than those in burn injury group[(80.62±2.16)%, (82.62±1.93)%, (80.32±3.05)%, (81.53±2.79)%], the difference was statistically significant(t=6.553, 4.465, 3.286, 7.420, all P values were less than 0.05). The levels of alanine aminotransferase (96.36±6.56)U/L, creatinine (79.35±4.16)μmol/L, diamine oxidase (78.54±5.23)U/L, creatine kinase MB isoenzyme (3712.64±309.45)U/L in sodium butyrate group were significantly lower than those in burn injury group [(113.54±7.41)U/L, (90.34±5.37)μmol/L, (92.34±5.34)U/L, (5264.46±351.62)U/L], the difference was statistically significant (t=4.910, 4.576, 5.222, 9.370, all P values were less than 0.05).

Conclusion

Sodium butyrate has significant protective effects on vital organs in rats by improving the visceral blood flow and tissue edema after extraordinarily severe burn injury.

Key words: Histone deacetylase inhibitors, Burns, Edema, Sodium butyrate, Organ function, Visceral blood flow
表1 各组大鼠伤后各时刻点肝、肾及小肠黏膜血流量情况(n＝8，BPU, ±s)
组别 肝 肾 小肠
伤后3 h 伤后6 h 伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 142.54±10.23 146.46±12.32 146.36±11.35 142.64±11.53 132.36±13.54 136.35±12.56
烧伤组 77.34±11.34 51.72±10.54 80.71±10.53 58.34±9.34 59.64±11.82 31.56±12.72
丁酸钠组 83.54±10.72 65.36±11.79 93.56±11.56 66.75±10.35 84.65±12.64 62.65±12.56
t1值 12.074 16.527 11.993 16.068 11.443 16.580
P1值 <0.05 <0.05 <0.05 <0.05 <0.05 <0.05
t2值 1.123 2.439 2.324 1.706 4.087 4.919
P2值 >0.05 <0.05 <0.05 >0.05 <0.05 <0.05
表2 各组大鼠伤后各时刻点ALT、Cr、CK- MB及DAO的变化(n＝8，±s)
组别 ALT (U/L) Cr (μmol/L)
伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 37.53±3.32 39.62±4.31 38.63±4.32 41.34±3.41
烧伤组 85.53±5.33 113.54±7.41 59.54±5.26 90.34±5.37
丁酸钠组 80.54±4.62 96.36±6.56 54.35±4.67 79.35±4.16
t1值 21.620 24.389 8.689 21.787
P1值 <0.05 <0.05 <0.05 <0.05
t2值 2.000 4.910 2.087 4.576
P2值 >0.05 <0.05 >0.05 <0.05
组别 CK- MB (U/L) DAO (U/L)
伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 2895.35±353.62 2912.45±403.53 23.54±3.43 22.46±4.510
烧伤组 3676.32±259.65 5264.46±351.62 71.43±5.23 92.34±5.34
丁酸钠组 2794.56±291.54 3712.64±309.45 66.65±4.69 78.54±5.23
t1值 5.035 12.429 21.657 28.277
P1值 <0.05 <0.05 <0.05 <0.05
t2值 6.388 9.370 1.924 5.222
P2值 <0.05 <0.05 >0.05 <0.05
表3 各组大鼠伤后各时刻点心、肺、肝、肾及小肠含水率的变化(n＝8，%，±s)
组别 心 肺 肝
伤后3 h 伤后6 h 伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 70.54±1.34 71.02±1.82 66.73±1.73 67.41±1.94 68.64±1.54 67.91±1.73
烧伤组 74.45±1.62 78.43±1.73 73.56±1.69 80.62±2.16 74.45±1.61 82.62±1.93
丁酸钠组 72.35±1.93 76.81±2.13 69.56±1.83 73.72±2.05 73.53±1.56 78.41±1.84
t1值 5.260 8.346 7.987 12.869 7.376 16.052
P1值 <0.05 <0.05 <0.05 <0.05 <0.05 <0.05
t2值 2.357 1.669 4.541 6.553 1.160 4.465
P2值 <0.05 >0.05 <0.05 <0.05 >0.05 <0.05
组别 肾 小肠
伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 70.46±1.73 70.83±1.57 60.34±2.75 61.62±2.63
烧伤组 74.23±2.56 80.32±3.05 72.54±2.93 81.53±2.79
丁酸钠组 73.51±1.63 75.64±2.63 63.75±2.58 70.53±3.13
t1值 3.451 7.824 8.587 14.687
P1值 <0.05 <0.05 <0.05 <0.05
t2值 0.671 3.286 6.368 7.420
P2值 >0.05 <0.05 <0.05 <0.05
[1]
胡森，盛志勇. 重视战、创(烧)伤休克现场非常规救治技术研究[J]. 解放军医学杂志，2011, 36(1): 5-7.
[2]
Fessler EB, Chibane FL, Wang Z, et al. Potential roles of HDAC inhibitors in mitigating ischemia- induced brain damage and facilitating endogenous regeneration and recovery [J]. Curr Pharm Des, 2013, 19(28): 5105-5120.
[3]
Granger A, Abdullah I, Huebner F, et al. Histone deacetylase inhibition reduces myocardial ischemia- reperfusion injury in mice [J]. FASEB J, 2008, 22(10): 3549-3560.
[4]
Gonzales ER, Chen H, Munuve RM, et al. Hepatoprotection and lethality rescue by histone deacetylase inhibitor valproic acid in fatal hemorrhagic shock[J]. J Trauma, 2008, 65(3): 554-565.
[5]
Cianciolo CC, Skrypnyk NI, Brilli LL, et al. Histone deacetylase inhibitor enhances recovery after AKI[J]. J Am Soc Nephrol, 2013, 24(6): 943-953.
[6]
Sun J, Wang F, Li H, et al. Neuroprotective Effect of Sodium Butyrate against Cerebral Ischemia/Reperfusion Injury in Mice[J]. Biomed Res Int, 2015, 2015: 395895.
[7]
Hu X, Zhang K, Xu C, et al. Anti- inflammatory effect of sodium butyrate preconditioning during myocardial ischemia reperfusion[J]. Exp Ther Med, 2014, 8(1): 229-232.
[8]
高明娟，刘维维，王晓娜，等. 丙酮酸钠羟乙基淀粉对50%总体表面积Ⅲ度烧伤大鼠肾血流量和肾功能的影响[J/CD]. 中华损伤与修复杂志：电子版，2015, 10(5): 388-394.
[9]
王洁，张心怡，郝彩丽，等. 组蛋白去乙酰化酶抑制剂的研究进展[J]. 现代生物医学进展，2014, 14(14): 2783-2785.
[10]
郑金光，胡森，李芳菲，等. 伏立诺他对50% TBSA Ⅲ度烫伤大鼠脏器功能及血流量的影响[J]. 感染、炎症、修复，2014, 15(4): 195-198.
[11]
王艳冰，任素萍，王庆军，等. 新型组蛋白去乙酰化酶抑制剂对缺氧损伤心肌细胞的保护作用研究[J]. 军事医学，2015, 39(1): 30-35.
[12]
Luo HM, Hu S, Bai HY, et al. Valproic acid treatmet attenuates caspase- 3 activation and improves survival following lethal burn injury in a rodent model[J]. J Burn Care Res, 2014, 35(2): e93-98.
[13]
Zhang L, Wan J, Jiang R, et al. Protective effects of trichostatin A on liver injury in septic mice[J]. Hepatol Res, 2009, 39(9): 931-938.
[14]
Gonzales E, Chen H, Munuve R, et al. Valproic acid prevents hemorrhage- associated lethality and affects the acetylation pattern of cardiac histones[J]. Shock, 2006, 25(4): 395-401.
[15]
Kilgore M, Miller CA, Fass DM, et al. Inhibitors of class 1 histone deacetylases reverse contextual memory deficits in a mouse model of Alzheimer′s disease[J]. Neuropsychopharmacology, 2010, 35(4): 870-880.
[16]
Shin H, Lee YS, Lee YC. Sodium butyrate- induced DAPK- mediated apoptosis in human gastric cancer cells[J]. Oncol Rep, 2012, 27(4): 1111-1115.
[17]
Liang X, Wang RS, Wang F, et al. Sodium butyrate protects against severe burn- induced remote acute lung injury in rats[J]. PloS one, 2013, 8(7): 191-195.
[18]
Han X, Song H, Wang Y, et al. Sodium butyrate protects the intestinal barrier function in peritonitic mice[J]. Int J Clin Exp Med, 2015, 8(3): 4000-4007.
[19]
Bates DO. Vascular endothelial growth factors and vascular permeability[J]. Cardiovasc Res, 2010, 87(2): 262-271.
[20]
Hur J, Yang HT, Chun W, et al. Inflammatory cytokines and their prognostic ability in cases of majorburninjury[J]. Ann Lab Med, 2015, 35(1)：105-110.
[21]
Sawa H, Murakami H, Ohshima Y, et al. Histone deacetylase inhibitors such as sodium butyrate and trichostatin A inhibit vascular endothelial growth factor (VEGF) secretion from human glioblastoma cells[J]. Brain Tumor Pathol, 2002, 19(2)：77-81.
[1] 王子杨, 杨文利, 李栋军, 陈伟, 赵琦, 李逸丰, 崔蕊, 沈琳, 刘倩, 魏串串. 高频线阵探头对眼球壁的临床观察[J/OL]. 中华医学超声杂志（电子版）, 2024, 21(06): 580-584.
[2] 李煜, 王鹏, 陆翮, 冯蓉琴, 韩军涛. 采用低频脉冲电刺激治疗深Ⅱ度烧伤创面的临床观察[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 474-478.
[3] 彭玲, 吴红, 宛仕勇, 陈斓, 叶子青, 周静. 胶原酶软膏联合水胶体敷料应用于深Ⅱ度烧伤创面治疗的效果观察[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 511-516.
[4] 林同辉, 杨卫玺. 股前外侧穿支皮瓣在电烧伤治疗中应用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 526-530.
[5] 王宏宇, 巴特, 黄瑞娟, 陈强, 闫增强. 亲属头皮加自体头皮混合移植接力在大面积深度烧伤创面修复中的应用[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 554-554.
[6] 张嘉炜, 王瑞, 张克诚, 易磊, 周增丁. 烧烫伤创面深度智能检测模型P-YOLO的建立及测试效果[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(05): 379-385.
[7] 毛书雷, 张元海, 王杰, 倪良方, 王新刚, 邹雁, 王荣娟, 吴军梅, 张建芬. 区域性静脉灌注葡萄糖酸钙治疗手指氢氟酸烧伤的临床疗效和安全性分析[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(05): 386-392.
[8] 孙俊锋, 涂家金, 付丹, 蒋满香, 刘金晶, 崔乃硕. 手部烧伤瘢痕挛缩畸形整形术后综合康复联合点阵二氧化碳激光治疗的临床效果[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(05): 411-415.
[9] 杨新园, 王淑君, 何成, 宋喜鹤, 刘丽芸. 预防与处理危重烧伤患者经外周静脉穿刺置入中心静脉导管堵塞的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(05): 443-446.
[10] 孙勇, 彭曦. 重视烧伤创面愈合中的葡萄糖代谢以优化营养治疗策略[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 277-281.
[11] 张锦丽, 席毛毛, 褚志刚, 栾夏刚, 陈诺, 王德运, 谢卫国. 大面积烧伤患者发生早期急性肾损伤的危险因素分析[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 282-287.
[12] 温春泉, 陈欣, 尹凯, 赵筱卓, 张琮, 程琳, 陈辉. 旋肩胛动脉穿支皮瓣在烧伤后重度腋窝瘢痕挛缩畸形整形修复治疗中的应用[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 294-298.
[13] 陈宏泽, 刘晟, 陈旭林. 水动力清创系统在大面积深Ⅱ度烧伤创面清创中的应用效果[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 314-318.
[14] 张庆庆, 曲毅睿, 王淑君, 孙凤, 侯晓敏, 耿丽, 孙天骏. 北京市某三甲医院急诊成人烧烫伤的流行病学特征[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 324-328.
[15] 王旭颖, 王玮, 李贵刚. 把握内眼术后角膜水肿行角膜内皮移植术的时机[J/OL]. 中华眼科医学杂志(电子版), 2024, 14(04): 193-199.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号