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中华损伤与修复杂志(电子版) ›› 2016, Vol. 11 ›› Issue (02) : 90 -95. doi: 10.3877/cma.j.issn.1673-9450.2016.02.003

所属专题： 文献；

论著

丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响
唐富波1, 张文华2, 李雨梦2, 李静远2, 刘锐3, 胡森2, 白晓东4,†()   
  1. 1. 100039　北京，武警总医院烧伤整形科；300309　天津，武警后勤学院
    2. 100048　北京，解放军总医院第一附属医院全军创伤修复与组织再生重点实验室暨皮肤损伤修复与组织再生北京市重点实验室
    3. 150040　哈尔滨市第五医院烧伤整形科
    4. 100039　北京，武警总医院烧伤整形科
  • 收稿日期:2015-12-10 出版日期:2016-04-01
  • 通信作者: 白晓东
  • 基金资助:
    国家自然科学基金(81471872); 国家发改委卫星示范专项(发改办高技（2013）2140号)

Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury

Fubo Tang1, Wenhua Zhang2, Yumeng Li2, Jingyuan Li2, Rui Liu3, Sen Hu2, Xiaodong Bai4,†()   

  1. 1. Department of Burn and Plastic Surgery, the General Hospital of Armed Police Forces, Beijing 100039, China; Logistics University of Chinese People′s Armed Police Forces, Tianjin 300309, China
    2. Laboratory for Shock and Multiple Organ Dysfunction, Key Research Laboratory of Tissue Repair and Regeneration of People′s Liberation Army, and Beijing Key Research Laboratory of Skin Injury and Repair Regeneration, the First Hospital Affiliated to General Hospital of People′s Liberation Army, Beijing 100048, China
    3. Department of Burn and Plastic Surgery, the Fifth Hospital of Harbin, Harbin 150040, China
    4. Department of Burn and Plastic Surgery, the General Hospital of Armed Police Forces, Beijing 100039, China
  • Received:2015-12-10 Published:2016-04-01
  • Corresponding author: Xiaodong Bai
  • About author:
    Corresponding author: Bai Xiaodong, Email:
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引用本文：

唐富波, 张文华, 李雨梦, 李静远, 刘锐, 胡森, 白晓东. 丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响[J]. 中华损伤与修复杂志(电子版), 2016, 11(02): 90-95.

Fubo Tang, Wenhua Zhang, Yumeng Li, Jingyuan Li, Rui Liu, Sen Hu, Xiaodong Bai. Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2016, 11(02): 90-95.

目的

研究丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响。

方法

雄性SD大鼠48只，体重(250±20)g，随机数字表法分为假伤组、烧伤组和丁酸钠组，每组16只。丁酸钠组与烧伤组采用沸水烫大鼠背部15 s、腹部8 s，造成50%TBSAⅢ度烧伤，伤后分别立即腹腔注射丁酸钠(400 mg/kg)与等体积0.9%氯化钠溶液。假伤组37℃温水浸泡后腹腔注射等体积0.9%氯化钠溶液。伤后3 h和6 h采用激光多普勒血流仪测定肝、肾、小肠黏膜血流量；腹主动脉取血检测血清谷丙转氨酶(ALT)、肌酐(Cr)、肌酸激酶同工酶(CK- MB)和二胺氧化酶(DAO)；处死大鼠取脏器组织采用干湿重法检测心、肺、肝、肾和小肠含水率。

结果

丁酸钠组伤后3 h肾和小肠黏膜血流量[(93.56±11.56)、(84.65±12.64)BPU]分别高于烧伤组[(80.71±10.53)、(59.64±11.82)BPU]，差异均有统计学意义(t＝2.324、4.087，P值均小于0.05)；心、肺、小肠含水率[(72.35±1.93)%、(69.56±1.83)%、(63.75±2.58)%]以及CK- MB水平(2794.56±291.54)U/L分别低于烧伤组(74.45±1.62)%、(73.56±1.69)%、(72.54±2.93)%、(3676.32±259.65)U/L，差异均有统计学意义(t＝2.357、4.541、6.368、6.388，P值均小于0.05)。丁酸钠组伤后6 h肝和小肠黏膜血流量[(65.36±11.79)、(62.65±12.56)BPU]分别高于烧伤组[(51.72±10.54)、(31.56±12.72)BPU]，差异均有统计学意义(t＝2.439、4.919，P值均小于0.05)；肺、肝、肾和小肠含水率[(73.72±2.05)%、(78.41±1.84)%、(75.64±2.63)%、(70.53±3.13)%]分别低于烧伤组[(80.62±2.16)%、(82.62±1.93)%、(80.32±3.05)%、(81.53±2.79)%]，差异均有统计学意义(t＝6.553、4.465、3.286、7.420，P值均小于0.05)；丁酸钠组血浆ALT(96.36±6.56)U/L、Cr(79.35±4.16)μmol/L、DAO(78.54±5.23)U/L、CK- MB(3712.64±309.45)U/L分别低于烧伤组[(113.54±7.41)U/L、(90.34±5.37)μmol/L、(92.34±5.34)U/L、(5264.46±351.62)U/L]，差异均有统计学意义(t＝4.910、4.576、5.222、9.370，P值均小于0.05)。

结论

丁酸钠能够增加特重度烧伤大鼠腹腔脏器血流量，减轻组织水肿，改善脏器功能，对特重度烧伤引起的重要脏器损害具有一定的保护作用。

关键词: 组蛋白脱乙酰基酶抑制剂, 烧伤, 水肿, 丁酸钠, 脏器功能, 血流量
Objective

To investigate the effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury.

Methods

Forty-eight male SD rats whose average weight were (250±20) grams were randomly divided into sham group, burn injury group and sodium butyrate group (n=16). Rats of burn injury group and sodium butyrate group were subjected to extraordinarily severe burn injury by scalding the rats′ back 15 s and abdomen 8 s with boiling water. And then sodium butyrate solution (400 mg/kg) or same volume of 0.9% sodium chloride solution was intraperitoneally injected, respectively. While the same volume of 0.9% sodium chloride solution was intraperitoneally injected into rats of sham group after soaking in 37 ℃ warm water. At 3 and 6 hours after injury, the blood flow of liver, kidney and small intestine were measured by laser doppler flowmetry, and blood samples were drawn from abdominal aorta for measurement of parameters of organ function and the activity of diamine oxidase. The tissues samples of kidney, liver, heart, lung and small intestine were harvested for the measurement of tissue water content.

Results

Compared with burn injury group at 3 hours after injury, the blood flow of kidney and small intestine in sodium butyrate group were (93.56±11.56) BPU and (84.65±12.64)BPU, significantly higher than those in burn injury group[(80.71±10.53) BPU, (59.64±11.82)BPU], the difference was statistically significant(t=2.324, 4.087, all P values were less than 0.05). The water content of heart, lung and small intestine in sodium butyrate group were (72.35±1.93)%, (69.56±1.83)%, (63.75±2.58)%, and the level of creatine kinase MB isoenzyme was(2794.56±291.54)U/L, significantly lower than those in burn injury group[(74.45±1.62)%, (73.56±1.69)%, (72.54±2.93)%] and (3676.32±259.65)U/L, the difference was statistically significant(t=2.357, 4.541, 6.368, 6.388, all P values were less than 0.05). Compared with burn injury group at 6 hours after injury, the blood flow of liver and small intestine in sodium butyrate group were (65.36±11.79) BPU, (62.65±12.56)BPU, significantly higher than those in burn injury group[(51.72±10.54) BPU, (31.56±12.72)BPU], the difference was statistically significant(t=2.439, 4.919, all P values were less than 0.05). The water content of lung, liver, kidney and small intestine in sodium butyrate group were (73.72±2.05)%, (78.41±1.84)%, (75.64±2.63)%, (70.53±3.13)%, significantly lower than those in burn injury group[(80.62±2.16)%, (82.62±1.93)%, (80.32±3.05)%, (81.53±2.79)%], the difference was statistically significant(t=6.553, 4.465, 3.286, 7.420, all P values were less than 0.05). The levels of alanine aminotransferase (96.36±6.56)U/L, creatinine (79.35±4.16)μmol/L, diamine oxidase (78.54±5.23)U/L, creatine kinase MB isoenzyme (3712.64±309.45)U/L in sodium butyrate group were significantly lower than those in burn injury group [(113.54±7.41)U/L, (90.34±5.37)μmol/L, (92.34±5.34)U/L, (5264.46±351.62)U/L], the difference was statistically significant (t=4.910, 4.576, 5.222, 9.370, all P values were less than 0.05).

Conclusion

Sodium butyrate has significant protective effects on vital organs in rats by improving the visceral blood flow and tissue edema after extraordinarily severe burn injury.

Key words: Histone deacetylase inhibitors, Burns, Edema, Sodium butyrate, Organ function, Visceral blood flow
表1 各组大鼠伤后各时刻点肝、肾及小肠黏膜血流量情况(n＝8，BPU, ±s)
组别 肝 肾 小肠
伤后3 h 伤后6 h 伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 142.54±10.23 146.46±12.32 146.36±11.35 142.64±11.53 132.36±13.54 136.35±12.56
烧伤组 77.34±11.34 51.72±10.54 80.71±10.53 58.34±9.34 59.64±11.82 31.56±12.72
丁酸钠组 83.54±10.72 65.36±11.79 93.56±11.56 66.75±10.35 84.65±12.64 62.65±12.56
t1值 12.074 16.527 11.993 16.068 11.443 16.580
P1值 <0.05 <0.05 <0.05 <0.05 <0.05 <0.05
t2值 1.123 2.439 2.324 1.706 4.087 4.919
P2值 >0.05 <0.05 <0.05 >0.05 <0.05 <0.05
表2 各组大鼠伤后各时刻点ALT、Cr、CK- MB及DAO的变化(n＝8，±s)
组别 ALT (U/L) Cr (μmol/L)
伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 37.53±3.32 39.62±4.31 38.63±4.32 41.34±3.41
烧伤组 85.53±5.33 113.54±7.41 59.54±5.26 90.34±5.37
丁酸钠组 80.54±4.62 96.36±6.56 54.35±4.67 79.35±4.16
t1值 21.620 24.389 8.689 21.787
P1值 <0.05 <0.05 <0.05 <0.05
t2值 2.000 4.910 2.087 4.576
P2值 >0.05 <0.05 >0.05 <0.05
组别 CK- MB (U/L) DAO (U/L)
伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 2895.35±353.62 2912.45±403.53 23.54±3.43 22.46±4.510
烧伤组 3676.32±259.65 5264.46±351.62 71.43±5.23 92.34±5.34
丁酸钠组 2794.56±291.54 3712.64±309.45 66.65±4.69 78.54±5.23
t1值 5.035 12.429 21.657 28.277
P1值 <0.05 <0.05 <0.05 <0.05
t2值 6.388 9.370 1.924 5.222
P2值 <0.05 <0.05 >0.05 <0.05
表3 各组大鼠伤后各时刻点心、肺、肝、肾及小肠含水率的变化(n＝8，%，±s)
组别 心 肺 肝
伤后3 h 伤后6 h 伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 70.54±1.34 71.02±1.82 66.73±1.73 67.41±1.94 68.64±1.54 67.91±1.73
烧伤组 74.45±1.62 78.43±1.73 73.56±1.69 80.62±2.16 74.45±1.61 82.62±1.93
丁酸钠组 72.35±1.93 76.81±2.13 69.56±1.83 73.72±2.05 73.53±1.56 78.41±1.84
t1值 5.260 8.346 7.987 12.869 7.376 16.052
P1值 <0.05 <0.05 <0.05 <0.05 <0.05 <0.05
t2值 2.357 1.669 4.541 6.553 1.160 4.465
P2值 <0.05 >0.05 <0.05 <0.05 >0.05 <0.05
组别 肾 小肠
伤后3 h 伤后6 h 伤后3 h 伤后6 h
假伤组 70.46±1.73 70.83±1.57 60.34±2.75 61.62±2.63
烧伤组 74.23±2.56 80.32±3.05 72.54±2.93 81.53±2.79
丁酸钠组 73.51±1.63 75.64±2.63 63.75±2.58 70.53±3.13
t1值 3.451 7.824 8.587 14.687
P1值 <0.05 <0.05 <0.05 <0.05
t2值 0.671 3.286 6.368 7.420
P2值 >0.05 <0.05 <0.05 <0.05
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