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中华损伤与修复杂志(电子版) ›› 2026, Vol. 21 ›› Issue (03) : 229 -235. doi: 10.3877/cma.j.issn.1673-9450.2026.03.010

综述

重组人Ⅲ型胶原蛋白在皮肤创面与瘢痕中的应用
曾妍1, 朱遇安1, 陶紫涵1, 金剑2, 刘青松2, 朱世辉1,2,()   
  1. 1 200433 上海,海军军医大学第一附属医院烧伤外科
    2 200127 上海交通大学医学院附属上海儿童医学中心烧伤整复外科
  • 收稿日期:2026-02-02 出版日期:2026-06-01
  • 通信作者: 朱世辉

Application of recombinant human type Ⅲ collagen in skin wounds and scars

Yan Zeng1, Yu'an Zhu1, Zihan Tao1, Jian Jin2, Qingsong Liu2, Shihui Zhu1,2,()   

  1. 1 Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
    2 Department of Burn and Plastic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
  • Received:2026-02-02 Published:2026-06-01
  • Corresponding author: Shihui Zhu
引用本文:

曾妍, 朱遇安, 陶紫涵, 金剑, 刘青松, 朱世辉. 重组人Ⅲ型胶原蛋白在皮肤创面与瘢痕中的应用[J/OL]. 中华损伤与修复杂志(电子版), 2026, 21(03): 229-235.

Yan Zeng, Yu'an Zhu, Zihan Tao, Jian Jin, Qingsong Liu, Shihui Zhu. Application of recombinant human type Ⅲ collagen in skin wounds and scars[J/OL]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2026, 21(03): 229-235.

Ⅲ型胶原蛋白(ColⅢ)是细胞外基质(ECM)的关键成分,对维持皮肤结构完整性与促进修复至关重要。传统动物源ColⅢ存在来源差异、免疫原性风险及批次差异等问题。基因工程生产的重组人Ⅲ型胶原蛋白(rhColⅢ)与天然ColⅢ高度同源,且克服了动物源风险。利用rhColⅢ的生物活性,可将其制备成电纺纳米纤维膜、水凝胶、微针贴片及游离rhColⅢ链等多种材料形式,以促进创面愈合,并减少瘢痕形成。rhColⅢ可能通过干预转化生长因子-β(TGF-β)/Smad信号通路下调促纤维化基因表达,或经尿激酶型纤溶酶原激活物受体相关蛋白(uPARAP)/内吞胶原蛋白受体180(Endo180)内吞途径进入成纤维细胞,作为合成ColⅢ的原料,直接调控ColⅠ/ColⅢ比例,从而促进创面修复。此外,该文探讨了表达系统与化学修饰对rhColⅢ结构与功能的影响,并从材料设计、机制研究、陈旧性瘢痕干预及临床转化等角度对未来研究提出建议。

Type Ⅲ collagen (ColⅢ) is a key component of the extracellular matrix (ECM) and is crucial for maintaining the structural integrity of the skin and promoting repair. Traditional animal-derived ColⅢ has issues such as source variations, immunogenicity risks, and batch differences. Recombinant human type Ⅲ collagen (rhColⅢ), produced through genetic engineering, is highly homologous to natural ColⅢ and overcomes the risks associated with animal sources. Utilizing the biological activity of rhColⅢ, it can be prepared into various material forms such as electrospun nanofiber membranes, hydrogels, microneedle patches, and free rhColⅢ chains to promote wound healing and reduce scar formation. rhColⅢ may downregulate the expression of fibrotic genes by interfering with the transforming growth factor-β (TGF-β)/Smad signaling pathways, or enter fibroblasts through the urokinase-type plasminogen activator receptor-associated protein (uPARAP)/endocytic receptor 180 (Endo180) endocytic pathway as raw materials for synthesizing ColⅢ, directly regulating the ratio of Col Ⅰ/ColⅢ, thereby promoting wound healing. Additionally, this review explores the effects of expression systems and chemical modifications on the structure and function of rhColⅢ, and proposes suggestions for future research from perspectives such as material design, mechanism study, intervention of old scars, and clinical translation.

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