To investigate the effet of ulinastatin on pulmonary tissue inflammatory mediators, vistal vasopermeability and tissue water content after moderate burn-blast combined injury in rats.

Eighty male Sprague-Dawley rats were randomly divided into the control group and ulinastatin group. A model with moderate burn-blast combined injury, was produced in both groups. Immediately after injury rats were intravenously given either 1 mL sodium chloride solution in control group or 1 mL sodium chloride solution containing ulinastatin in ulinastatin group (40 000 U/kg). The content of tumor necrosis factor-α, interleukin (IL) - 6 and interleukin (IL) - 8 were detected by enzyme linked immuno sorbent assay (ELISA) at 6 hours and 24 hours after injury. The vasopermeability was detected by the method of evans blue and the rates of tissue water content in lung were detected by the dry /wet weight. Measurement data between groups was analyzed by t test.

Compared with control group after 6 h, the inflammatory mediators of TNF-α、IL- 6 and IL- 8 in ulinastatin group were (2103±168)pg/mL, (2315±185)pg/mL and(1827±134)pg/mL, significantly lower than those(2912±184)pg/mL, (2793±223)pg/mL and (2178±145)pg/mL in control group, the difference was statistically significant(t=6.114, 8.123, 7.347, P<0.05). Compared with 24 hours, the inflammatory mediators of TNF-α、IL - 6 and IL - 8 in ulinastatin group were (1235±86)pg/mL, (1093±98)pg/mL and(973±77)pg/mL, even more significantly lower than those(1853±166)pg/mL, (2152±172)pg/mL and(1807±126)pg/mL in control group, the difference was statistically significant(t=5.176, 7.043, 5.732, P<0.05). Compared with control group after 6 hours and 24 hours, the visceral content of evans blue in ulinastatin group were (63.9±3.3), (59.8±3.2)μg/g, significantly lower than those(78.2±3.7), (76.3±3.5) μg/g in control group, the difference was statistically significant(t=7.023, 7.382, P<0.05). Compared with control group after 6 hours and 24 hours, the rate of tissue water content in lung in ulinastatin group were (76.3±1.45)%、(73.8±1.35)%, obviously lower than those (79.8±1.52)%、(78.3±1.47)% in enzyme linked immuno sorbent assay, the difference was statistically significant(t=2.041, 2.758, P<0.05).

The results indicate that ulinastatin significantly alleviate systemic inflammation, inhibit visceral vasopermeability and tissue edema, and it can be used to be early period therapy of burn shock and improve the survival rate.