Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury

doi:10.3877/cma.j.issn.1673-9450.2016.02.003

Abstract

Abstract:

Objective

To investigate the effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury.

Methods

Forty-eight male SD rats whose average weight were (250±20) grams were randomly divided into sham group, burn injury group and sodium butyrate group (n=16). Rats of burn injury group and sodium butyrate group were subjected to extraordinarily severe burn injury by scalding the rats′ back 15 s and abdomen 8 s with boiling water. And then sodium butyrate solution (400 mg/kg) or same volume of 0.9% sodium chloride solution was intraperitoneally injected, respectively. While the same volume of 0.9% sodium chloride solution was intraperitoneally injected into rats of sham group after soaking in 37 ℃ warm water. At 3 and 6 hours after injury, the blood flow of liver, kidney and small intestine were measured by laser doppler flowmetry, and blood samples were drawn from abdominal aorta for measurement of parameters of organ function and the activity of diamine oxidase. The tissues samples of kidney, liver, heart, lung and small intestine were harvested for the measurement of tissue water content.

Results

Compared with burn injury group at 3 hours after injury, the blood flow of kidney and small intestine in sodium butyrate group were (93.56±11.56) BPU and (84.65±12.64)BPU, significantly higher than those in burn injury group[(80.71±10.53) BPU, (59.64±11.82)BPU], the difference was statistically significant(t=2.324, 4.087, all P values were less than 0.05). The water content of heart, lung and small intestine in sodium butyrate group were (72.35±1.93)%, (69.56±1.83)%, (63.75±2.58)%, and the level of creatine kinase MB isoenzyme was(2794.56±291.54)U/L, significantly lower than those in burn injury group[(74.45±1.62)%, (73.56±1.69)%, (72.54±2.93)%] and (3676.32±259.65)U/L, the difference was statistically significant(t=2.357, 4.541, 6.368, 6.388, all P values were less than 0.05). Compared with burn injury group at 6 hours after injury, the blood flow of liver and small intestine in sodium butyrate group were (65.36±11.79) BPU, (62.65±12.56)BPU, significantly higher than those in burn injury group[(51.72±10.54) BPU, (31.56±12.72)BPU], the difference was statistically significant(t=2.439, 4.919, all P values were less than 0.05). The water content of lung, liver, kidney and small intestine in sodium butyrate group were (73.72±2.05)%, (78.41±1.84)%, (75.64±2.63)%, (70.53±3.13)%, significantly lower than those in burn injury group[(80.62±2.16)%, (82.62±1.93)%, (80.32±3.05)%, (81.53±2.79)%], the difference was statistically significant(t=6.553, 4.465, 3.286, 7.420, all P values were less than 0.05). The levels of alanine aminotransferase (96.36±6.56)U/L, creatinine (79.35±4.16)μmol/L, diamine oxidase (78.54±5.23)U/L, creatine kinase MB isoenzyme (3712.64±309.45)U/L in sodium butyrate group were significantly lower than those in burn injury group [(113.54±7.41)U/L, (90.34±5.37)μmol/L, (92.34±5.34)U/L, (5264.46±351.62)U/L], the difference was statistically significant (t=4.910, 4.576, 5.222, 9.370, all P values were less than 0.05).

Conclusion

Sodium butyrate has significant protective effects on vital organs in rats by improving the visceral blood flow and tissue edema after extraordinarily severe burn injury.

Key words: Histone deacetylase inhibitors, Burns, Edema, Sodium butyrate, Organ function, Visceral blood flow

Fubo Tang, Wenhua Zhang, Yumeng Li, Jingyuan Li, Rui Liu, Sen Hu, Xiaodong Bai. Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2016, 11(02): 90-95.

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References 21

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组别	肝		肾		小肠
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	142.54±10.23	146.46±12.32	146.36±11.35	142.64±11.53	132.36±13.54	136.35±12.56
烧伤组	77.34±11.34	51.72±10.54	80.71±10.53	58.34±9.34	59.64±11.82	31.56±12.72
丁酸钠组	83.54±10.72	65.36±11.79	93.56±11.56	66.75±10.35	84.65±12.64	62.65±12.56
t₁值	12.074	16.527	11.993	16.068	11.443	16.580
P₁值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05
t₂值	1.123	2.439	2.324	1.706	4.087	4.919
P₂值	>0.05	<0.05	<0.05	>0.05	<0.05	<0.05

组别	肝		肾		小肠
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	142.54±10.23	146.46±12.32	146.36±11.35	142.64±11.53	132.36±13.54	136.35±12.56
烧伤组	77.34±11.34	51.72±10.54	80.71±10.53	58.34±9.34	59.64±11.82	31.56±12.72
丁酸钠组	83.54±10.72	65.36±11.79	93.56±11.56	66.75±10.35	84.65±12.64	62.65±12.56
t₁值	12.074	16.527	11.993	16.068	11.443	16.580
P₁值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05
t₂值	1.123	2.439	2.324	1.706	4.087	4.919
P₂值	>0.05	<0.05	<0.05	>0.05	<0.05	<0.05

组别	ALT (U/L)		Cr (μmol/L)
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	37.53±3.32	39.62±4.31	38.63±4.32	41.34±3.41
烧伤组	85.53±5.33	113.54±7.41	59.54±5.26	90.34±5.37
丁酸钠组	80.54±4.62	96.36±6.56	54.35±4.67	79.35±4.16
t₁值	21.620	24.389	8.689	21.787
P₁值	<0.05	<0.05	<0.05	<0.05
t₂值	2.000	4.910	2.087	4.576
P₂值	>0.05	<0.05	>0.05	<0.05
组别	CK- MB (U/L)		DAO (U/L)
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	2895.35±353.62	2912.45±403.53	23.54±3.43	22.46±4.510
烧伤组	3676.32±259.65	5264.46±351.62	71.43±5.23	92.34±5.34
丁酸钠组	2794.56±291.54	3712.64±309.45	66.65±4.69	78.54±5.23
t₁值	5.035	12.429	21.657	28.277
P₁值	<0.05	<0.05	<0.05	<0.05
t₂值	6.388	9.370	1.924	5.222
P₂值	<0.05	<0.05	>0.05	<0.05

组别	ALT (U/L)		Cr (μmol/L)
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	37.53±3.32	39.62±4.31	38.63±4.32	41.34±3.41
烧伤组	85.53±5.33	113.54±7.41	59.54±5.26	90.34±5.37
丁酸钠组	80.54±4.62	96.36±6.56	54.35±4.67	79.35±4.16
t₁值	21.620	24.389	8.689	21.787
P₁值	<0.05	<0.05	<0.05	<0.05
t₂值	2.000	4.910	2.087	4.576
P₂值	>0.05	<0.05	>0.05	<0.05
组别	CK- MB (U/L)		DAO (U/L)
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	2895.35±353.62	2912.45±403.53	23.54±3.43	22.46±4.510
烧伤组	3676.32±259.65	5264.46±351.62	71.43±5.23	92.34±5.34
丁酸钠组	2794.56±291.54	3712.64±309.45	66.65±4.69	78.54±5.23
t₁值	5.035	12.429	21.657	28.277
P₁值	<0.05	<0.05	<0.05	<0.05
t₂值	6.388	9.370	1.924	5.222
P₂值	<0.05	<0.05	>0.05	<0.05

组别	心		肺		肝
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	70.54±1.34	71.02±1.82	66.73±1.73	67.41±1.94	68.64±1.54	67.91±1.73
烧伤组	74.45±1.62	78.43±1.73	73.56±1.69	80.62±2.16	74.45±1.61	82.62±1.93
丁酸钠组	72.35±1.93	76.81±2.13	69.56±1.83	73.72±2.05	73.53±1.56	78.41±1.84
t₁值	5.260	8.346	7.987	12.869	7.376	16.052
P₁值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05
t₂值	2.357	1.669	4.541	6.553	1.160	4.465
P₂值	<0.05	>0.05	<0.05	<0.05	>0.05	<0.05
组别	肾		小肠
组别	伤后3 h	伤后6 h	伤后3 h	伤后6 h
假伤组	70.46±1.73	70.83±1.57	60.34±2.75	61.62±2.63
烧伤组	74.23±2.56	80.32±3.05	72.54±2.93	81.53±2.79
丁酸钠组	73.51±1.63	75.64±2.63	63.75±2.58	70.53±3.13
t₁值	3.451	7.824	8.587	14.687
P₁值	<0.05	<0.05	<0.05	<0.05
t₂值	0.671	3.286	6.368	7.420
P₂值	>0.05	<0.05	<0.05	<0.05

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