Research progress of stem cell-derived exosomes in injury repair

doi:10.3877/cma.j.issn.1673-9450.2021.01.014

Abstract

Abstract:

After organ and tissue injury, the proliferation and differentiation of stem cells themselves play only a small role in the repair of injury, and their repair function is mainly played by the paracrine function of stem cells.Exosomes are nano-scale vesicles that are secreted out of the cell by exocytosis. After target cells absorb exosomes, their own functions will be regulated. Stem cell-derived exosomes achieve intercellular communication by delivering proteins, lipids, and microRNA (miRNA). The targeting and biological characteristics of stem cell-derived exosomes are determined by the level of miRNA that they carry. After the exosomes reach the target cells and undergo fusion, they are degraded and then expressed to change the gene expression of the target cells. In addition, the RNA and protein of stem cell-derived exosomes can also limit the development of damage through cell homing. This article will review the mechanism of stem cell-derived exosomes in wound healing, joint injury, fracture healing and heart injury.

Key words: Stem cells, Wound healing, Exosomes, microRNA, Injury repair

Fengrui Cui, Fang Li, Tiening Zhang, Quan Li. Research progress of stem cell-derived exosomes in injury repair[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(01): 71-73.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhssyxfzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1673-9450.2021.01.014

https://zhssyxfzz.cma-cmc.com.cn/EN/Y2021/V16/I01/71

References 35

1	Gauthier SA, Pérez-González R, Sharma A, et al. Enhanced exosome secretion in down syndrome brain - a protective mechanism to alleviate neuronal endosomal abnormalities[J]. Acta Neuropathol Commun, 2017, 5(1): 65.
2	Mobergslien A, Sioud M. Exosome-derived miRNAs and cellular miRNAs activate innate immunity[J]. J Innate Immun, 2014, 6(1): 105-110.
3	Katsuda T, Tsuchiya R, Kosaka N, et al. Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes[J]. Sci Rep, 2013, 3: 1197.
4	肖春红，冉曦，冉新泽. 干细胞治疗皮肤创伤的研究[J/CD]. 中华损伤与修复杂志(电子版), 2016, 11(5): 357-360.
5	Strong AL, Neumeister MW, Levi B. Stem cells and tissue engineering: Regeneration of the skin and its contents[J]. Clin Plast Surg, 2017, 44(3): 635-650.
6	曹胜军，王凌峰，巴特，等. 脂肪源性间充质干细胞的基础与临床应用研究进展[J]. 中华烧伤杂志，2017, 33(3): 184-189.
7	Zhang LX, Shen LL, Ge SH, et al. Systemic BMSC homing in the regeneration of pulp-like tissue and the enhancing effect of stromal cell-derived factor-1 on BMSC homing[J]. Int J Clin Exp Pathol, 2015, 8(9): 10261-10271.
8	Li T, Yan Y, Wang B, et al. Exosomes derived from human umbilical cord mesenchymal stem cells alleviate liver fibrosis[J]. Stem Cells Dev, 2013, 22(6): 845-854.
9	Rodriguez J, Boucher F, Lequeux C, et al. Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice[J]. Stem Cell Res Ther, 2015, 6: 241.
10	Vériter S, André W, Aouassar N, et al. Human adipose-derived mesenchymal stem cells in cell therapy: Safety and feasibility in different " hospital exemption" clinical applications[J]. PLoS One, 2015, 10(10): e0139566.
11	李全，崔凤瑞，巴特，等. 脂肪干细胞来源的外泌体促进肉芽组织来源的成纤维细胞增殖的初步研究[J/CD]. 中华损伤与修复杂志(电子版), 2019, 14(2): 91-96.
12	Quesenberry PJ, Dooner MS, Aliotta JM. Stem cell plasticity revisited: The continuum marrow model and phenotypic changes mediated by microvesicles[J]. Exp Hematol, 2010, 38(7): 581-592.
13	Fang S, Xu C, Zhang Y, et al. Umbilical cord-derived mesenchymal stem cell-derived exosomal microRNAs suppress myofibroblast differentiation by inhibiting the transforming growth factor-beta/ Smad2 pathway during wound healing[J]. Stem Cells Transl Med, 2016, 5(10): 1425-1439.
14	Kang T, Jones TM, Naddell C, et al. Adipose-derived stem cells induce angiogenesis via microvesicle transport of miRNA-31[J]. Stem Cells Transl Med, 2016, 5(4): 440-450.
15	Hu L, Wang J, Zhou X, et al. Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts[J]. Sci Rep, 2016, 6: 32993.
16	Toh WS, Lai RC, Hui JHP, et al. MSC exosome as a cell-free MSC therapy for cartilage regeneration: Implications for osteoarthritis treatment[J]. Semin Cell Dev Biol, 2017, 67: 56-64.
17	Nakamura Y, Inloes JB, Katagiri T, et al. Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling[J]. Mol Cell Biol, 2011, 31(14): 3019-3028.
18	Li J, Huang J, Dai L, et al. MiR-146a, an IL-1beta responsive miRNA, induces vascular endothelial growth factor and chondrocyte apoptosis by targeting Smad4[J]. Arthritis Res Ther, 2012, 14(2): R75.
19	Jones SW, Watkins G, Le Good N, et al. The identification of differentially expressed microRNA in osteoarthritic tissue that modulate the production of TNF-alpha and MMP13[J]. Osteoarthritis Cartilage, 2009, 17(4): 464-472.
20	Lai RC, Yeo RW, Lim SK. Mesenchymal stem cell exosomes[J]. Semin Cell Dev Biol, 2015, 40: 82-88.
21	Yan S, Wang M, Zhao J, et al. MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis[J]. Int J Mol Med, 2016, 38(1): 201-209.
22	Zhang HG, Liu C, Su K, et al. A membrane form of TNF-alpha presented by exosomes delays T cell activation-induced cell death[J]. J Immunol, 2006, 176(12): 7385-7393.
23	Hao ZC, Lu J, Wang SZ, et al. Stem cell-derived exosomes: A promising strategy for fracture healing[J]. Cell Prolif, 2017, 50(5): e12359.
24	Liu X, Li Q, Niu X, et al. Exosomes secreted from human-induced pluripotent stem cell-derived mesenchymal stem cells prevent osteonecrosis of the femoral head by promoting angiogenesis[J]. Int J Biol Sci, 2017, 13(2): 232-244.
25	Lamplot JD, Qin J, Nan G, et al. BMP9 signaling in stem cell differentiation and osteogenesis[J]. Am J Stem Cells, 2013, 2(1): 1-21.
26	Martins M, Ribeiro D, Martins A, et al. Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment[J]. Stem Cell Reports, 2016, 6(3): 284-291.
27	Inose H, Ochi H, Kimura A, et al. A microRNA regulatory mechanism of osteoblast differentiation[J]. Proc Natl Acad Sci U S A, 2009, 106(49): 20794-20799.
28	Xu JF, Yang GH, Pan XH, et al. Altered microRNA expression profile in exosomes during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells[J]. PLoS One, 2014, 9(12): e114627.
29	Furuta T, Miyaki S, Ishitobi H, et al. Mesenchymal stem cell-derived exosomes promote fracture healing in a mouse model[J]. Stem Cells Transl Med, 2016, 5(12): 1620-1630.
30	Bilal M, Haseeb A, Sher Khan MA. Intracoronary infusion of wharton′s jelly-derived mesenchymal stem cells: A novel treatment in patients of acute myocardial infarction[J]. J Pak Med Assoc, 2015, 65(12): 1369.
31	Pereg D, Cohen K, Mosseri M, et al. Incidence and expression of circulating cell free p53-related genes in acute myocardial infarction patients[J]. J Atheroscler Thromb, 2015, 22(9): 981-998.
32	Arslan F, Lai RC, Smeets MB, et al. Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury[J]. Stem Cell Res, 2013, 10(3): 301-312.
33	Suzuki T, Inoki K. Spatial regulation of the mTORC1 system in amino acids sensing pathway[J]. Acta Biochim Biophys Sin, 2011, 43(9): 671-679.
34	Barile L, Lionetti V, Cervio E, et al. Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction[J]. Cardiovasc Res, 2014, 103(4): 530-541.
35	Nakazato K, Naganuma W, Ogawa K, et al. Attenuation of ischemic myocardial injury and dysfunction by cardiac fibroblast-derived factors[J]. Fukushima J Med Sci, 2010, 56(1): 1-16.

[1]	Wen Yan, Xingwen Xie, Yubiao Gu, Ningbo Lei, Cheng Ma, Wenxia Yu, Yaxiong Gao, Lei Zhang. Research progress of microRNA and deep vein thrombosis after total knee arthroplasty [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2023, 17(06): 842-846.
[2]	Chen Luo, Kaican Zong, Shiying Li, Yingya Fu. MicroRNA-199a-3p participates in the immune response after Mycoplasma pneumoniae infection in children by regulating the expression of CD4⁺ T cells [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 569-574.
[3]	Jian Zhang, Xiaolong Liu, Tianjian Zha, Junjie Yao, Jie Wang. Effect of platelet-rich plasma combined with xenogeneic acellular dermal matrix in the repair of ischemic wound of diabetic foot [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 503-506.
[4]	Linian Han, Jun Wang. Advances in the treatment of radiation-induced skin injury [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 533-537.
[5]	Zihui Zhou, Gongchi Li, Binghui Li, Zhi Wang, Huizhen Liu, Hui Wang, Lijun Zou. Advances of autophagy in wound healing [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 542-546.
[6]	Xuefeng He, Shixin Zhao, Peishan Li, Hengdeng Liu, Julin Xie. Kunzea ericoides leaf extract promotes wound healing in rats by enhancing biological functions of dermal fibroblasts [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(05): 405-412.
[7]	Yamei Zhao, Bin Xie, Yan Chen, Jian Wu. Effect of antibiotic bone cement combined with vacuum sealing drainage for diabetic foot ulcers: a meta-analysis [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(05): 427-433.
[8]	Jiqiu Chen, Shihui Zhu. Clinical application status of skin stretching device [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(05): 451-453.
[9]	Xuyuan Chen, Shiyun Luo, Wenzhong Li, Yi Li. Analysis of risk factors for wound healing difficulties after operative treatment of adenogenic anal fistula [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 82-85.
[10]	Weiqiang Ma, Binlin Ma, Zhongyu Wu, Ying Zhang. The role of microRNA in the progression of triple-negative breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 111-114.
[11]	Chufeng Wang, An Jiang. Molecular diagnosis of primary liver cancer [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(05): 499-503.
[12]	Zhuo Liu, Hubin Duan. Application progress of bioelectric therapy in nerve injury repair [J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2023, 09(05): 257-260.
[13]	Qiang Fu, Liyuan Qin, Quanbo Li. Analysis of serum miR-15a level and significance in patients with neuropathic pain [J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2023, 13(05): 293-298.
[14]	Qiang Ma, Jun Li, Lijuan Gou. Expression levels of miR-21-3p and RUNX3 in severe acute pancreatitis and their predictive value to the progression of the disease [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(05): 337-341.
[15]	Yuan Yuan, Liangping Zhao, Zhihui Liu, Liping Zhang, Limei Tan, Mengqin Xia. Relationship between miR-25-3p and PTEN expression and pathological parameters in endometrial cancer [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(9): 1016-1020.

Please choose a citation manager

Content to export