Characteristics of annulus fibrosus-derived mesenchymal stem cells and research progress of its purification and screening methods

doi:10.3877/cma.j.issn.1673-9450.2021.04.014

Abstract

Abstract:

Annulus fibrosus is an important part of intervertebral disc, which plays a vital role in maintaining biomechanical stability and forming normal anatomical structure of intervertebral disc. Annulus fibrosus cell injury and apoptosis are the main causes of intervertebral disc degeneration. At present, the methods to repair annulus fibrosus mainly include genetic engineering, cell transplantation and tissue engineering. Previous studies have shown that annulus fibrosus-derived mesenchymal stem cells (AFMSC) can be isolated from annulus fibrosus tissue. However, the utilization of the stem cells isolated from the degenerated annulus fibrosus is restricted due to the difficulty in obtaining the annulus fibrosus specimen and the poor cell viability. In this paper, the proliferation characteristics, differentiation characteristics, separation and purification methods of AFMSC and the research progress in separation and purification are reviewed.

Key words: Mesenchymal stem cells, Stem cells, Intervertebral disk degeneration, Annulus fibrosus, Monoclonal culture

Shuguang Wang, Wenjie Zhang, Xinmin Feng, Liping Nan, Feng Wang, Lei Zhu, Tongchuan Cai, Dong Chen, Liang Zhang. Characteristics of annulus fibrosus-derived mesenchymal stem cells and research progress of its purification and screening methods[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2021, 16(04): 358-361.

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