Abstract:
Objective To investigate the therapeutic effect of human adipose-derived stem cells (ADSCs) sheet on full-thickness skin defect in BALB/c-nu nude mice. The sample number was 6 in each group at each time point. Data were statistically analyed with t test.
Methods Human ADSCs were isolated and cultured in vitro. Three layers of human ADSCs sheet were prepared. A 12 mm-diameter, round, full-thickness cutaneous wound was made on the back of 36 nude mice. The nude mice were divided into two groups, sheep acelluar dermal matrix (ADM) was used alone to cover the wound in control group, and ADSCs sheet combined with sheep ADM were used in ADSCs sheet group. On 7 days after surgery, the thickness of granulation tissue was measured. On 7, 12 and 17 days after surgery, the wound healing rate and epidermal growth factor (EGF) expression in wound tissue were compared between the two groups.
Results The cells isolated and cultured from adipose tissue were spindle shaped, arranged in whirlpool. Flow cytometry and differentiation experiments confirmed that the cells were ADSCs. After sheet formation culture, ADSCs formed membrane. On 7 days after surgery, the thickness of wound granulation tissue in ADSCs sheet group was (456.35±57.05)μm, which was significantly larger than (295.08±38.86)μm in control group(t=5.721, P<0.001). On 7, 12 and 17 days after surgery, the wound healing rates in ADSCs sheet group [(45.07±1.58)%, (83.29±1.54)%, (98.49±0.85)%] were significantly higher than those in control group [(29.99±0.90)%, (78.18±1.02)%, (92.38±0.54)%], the differences were statistically significant (t=20.294, 6.777, 14.733; P<0.001). On 7, 12 and 17 days after surgery, EGF expressions in wound tissue in ADSCs sheet group [(90.32±2.36)pg/ml, (107.84±1.82)pg/ml, (111.93±2.25)pg/ml] were significantly higher than those in control group [(81.14±2.15)pg/ml, (102.46±2.18)pg/ml, (106.14±2.10)pg/ml], with statistically significant difference(t=7.012, 4.621, 4.630; P<0.001).
Conclusion Human ADSCs sheet could accelerate wound healing in nude mice, its mechanism may be related to the increase of EGF expression, which promotes the growth of granulation tissue.
Key words:
Adipose-derived stem cells,
Cell sheet technology,
Wound healing
Shengjun Cao, Quan Li, Xue Fu, Tianxi Shao, Yanhua Zhou. Observation on the efficacy of human adipose-derived stem cells multilayer sheet promoting wound healing in nude mice[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2024, 19(04): 341-347.