Research progress of fibroblast reprogramming and wound repair

doi:10.3877/cma.j.issn.1673-9450.2024.02.015

Abstract

Abstract:

Skin wound is one of the most common clinical diseases, and the outcome of wound healing includes regenerative repair and fibrosis repair. As the leading form of adult tissue and organ repair, fibrosis repair, also known as scar repair, will not only lead to tissue and organ dysfunction of patients, affect the appearance and physical and mental health, but also increase the medical and economic burden of the country. Therefore, how to inhibit fibrosis repair and promote regenerative repairin wound healing, so as to further maintain the integrity and function of the injured skin has been a major problem of medicine. In recent years, the development of stem cells and reprogramming techniques has brought conceptual innovation to regenerative medicine. And fibroblast reprogramming provides a new technical support for regenerative repair of large area burn and severe trauma. In this paper, the biological characteristics of skin fibroblasts are summarized, and the recent research progress of fibroblast reprogramming in wound repair is reviewed.

Key words: Fibroblast, Cell reprogramming, Wound healing, Regenerative repair

Gaoyu Liu, Peng Luo, Chunmeng Shi. Research progress of fibroblast reprogramming and wound repair[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2024, 19(02): 176-179.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhssyxfzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1673-9450.2024.02.015

https://zhssyxfzz.cma-cmc.com.cn/EN/Y2024/V19/I02/176

References 45

[1]	Gurtner GC, Werner S, Barrandon Y, et al. Wound repair and regeneration[J]. Nature, 2008, 453(7193): 314-321.
[2]	Coentro JQ, Pugliese E, Hanley G, et al. Current and upcoming therapies to modulate skin scarring and fibrosis[J]. Adv Drug Deliv Rev, 2019, 146: 37-59.
[3]	Plikus MV, Guerrero-Juarez CF, Ito M, et al. Regeneration of fat cells from myofibroblasts during wound healing[J]. Science, 2017, 355(6326): 748-752.
[4]	Wang H, Yang Y, Liu J, et al. Direct cell reprogramming: approaches, mechanisms and progress[J]. Nat Rev Mol Cell Biol, 2021, 22(6): 410-424.
[5]	Tani H, Sadahiro T, Yamada Y, et al. Direct reprogramming improves cardiac function and reverses fibrosis in chronic myocardial infarction[J]. Circulation, 2023, 147(3): 223-238.
[6]	Mascharak S, desJardins-Park HE, Davitt MF, et al. Preventing engrailed-1 activation in fibroblasts yields wound regeneration without scarring[J]. Science, 2021, 372(6540): eaba2374.
[7]	Sandri G, Aguzzi C, Rossi S, et al. Halloysite and chitosan oligosaccharide nanocomposite for wound healing[J]. Acta Biomater, 2017, 57: 216-224.
[8]	刘雪婷，白春雨，关伟军，等. 表皮干细胞的生物学特性及其潜在应用[J]. 生物技术通报，2016 (1): 29-32.
[9]	李彬彬，孙培鸣，孙宏伟，等. 表皮干细胞研究进展[J]. 医学研究杂志，2021, 50(1): 156-159.
[10]	Kurita M, Araoka T, Hishida T, et al. In vivo reprogramming of wound-resident cells generates skin epithelial tissue[J]. Nature, 2018, 561(7722): 243-247.
[11]	Yang R, Zheng Y, Burrows M, et al. Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells[J]. Nat Commun, 2014, 5: 3071.
[12]	Sun X, Xiang J, Chen R, et al. Sweat gland organoids originating from reprogrammed epidermal keratinocytes functionally recapitulated damaged skin[J]. Adv Sci (Weinh), 2021, 8(22): e2103079.
[13]	Ji SF, Zhou LX, Sun ZF, et al. Small molecules facilitate single factor-mediated sweat gland cell reprogramming[J]. Mil Med Res, 2022, 9(1): 13.
[14]	Guan J, Wang G, Wang J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells[J]. Nature, 2022, 605(7909): 325-331.
[15]	Allanki S, Strilic B, Scheinberger L, et al. Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration[J]. Sci Adv, 2021, 7(37): eabg6497.
[16]	Mazini L, Rochette L, Admou B, et al. Hopes and limits of adipose-derived stem cells (ADSCs) and mesenchymal stem cells (MSCs) in Wound Healing[J]. Int J Mol Sci, 2020, 21(4): 1306.
[17]	杨玲玲，黄悦，王洪一，等. 脂肪干细胞抑制炎症对缓解兔耳增生性瘢痕形成效果研究[J]. 临床军医杂志，2022, 50(5): 503-506, 509.
[18]	Kim WS, Park BS, Sung JH, et al. Wound healing effect of adipose-derived stem cells: a critical role of secretory factors on human dermal fibroblasts[J]. J Dermatol Sci, 2007, 48(1): 15-24.
[19]	Nambu M, Kishimoto S, Nakamura S, et al. Accelerated wound healing in healing-impaired db/db mice by autologous adipose tissue-derived stromal cells combined with atelocollagen matrix[J]. Ann Plast Surg, 2009, 62(3): 317-321.
[20]	Jeong JH. Adipose stem cells and skin repair[J]. Curr Stem Cell Res Ther, 2010, 5(2): 137-140.
[21]	Shao Y, Chen QZ, Zeng YH, et al. All-trans retinoic acid shifts rosiglitazone-induced adipogenic differentiation to osteogenic differentiation in mouse embryonic fibroblasts[J]. Int J Mol Med, 2016, 38(6): 1693-1702.
[22]	Zhang LJ, Guerrero-Juarez CF, Hata T, et al. Innate immunity. Dermal adipocytes protect against invasive Staphylococcus aureus skin infection[J]. Science, 2015, 347(6217): 67-71.
[23]	Franz A, Wood W, Martin P. Fat body cells are motile and actively migrate to wounds to drive Repair and Prevent Infection[J]. Dev Cell, 2018, 44(4): 460-470.e3.
[24]	Kwon HH, Yang SH, Lee J, et al. Combination treatment with human adipose tissue stem cell-derived exosomes and fractional CO₂ laser for acne scars: a 12-week prospective, double-blind, randomized, split-face study[J]. Acta Derm Venereol, 2020, 100(18): adv00310.
[25]	谭景铭，周胤朴. 脂肪干细胞外泌体应用于瘢痕治疗的研究进展[J]. 医学研究生学报，2022, 35(6): 668-672.
[26]	Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J]. Cell, 2006, 126(4): 663-676.
[27]	Li D, Shu X, Zhu P, et al. Chromatin accessibility dynamics during cell fate reprogramming[J]. EMBO Rep, 2021, 22(2): e51644.
[28]	Xie X, Jankauskas R, Mazari AMA, et al. beta-actin regulates a heterochromatin landscape essential for optimal induction of neuronal programs during direct reprograming[J]. PLoS Genet, 2018, 14(12): e1007846.
[29]	Balmer P, Hariton WVJ, Sayar BS, et al. SUV39H2 epigenetic silencing controls fate conversion of epidermal stem and progenitor cells[J]. J Cell Biol, 2021, 220(4): e201908178.
[30]	Adachi K, Kopp W, Wu G, et al. Esrrb unlocks silenced enhancers for reprogramming to naive pluripotency[J]. Cell Stem Cell, 2018, 23(2): 266-275.e6.
[31]	Hernandez C, Wang Z, Ramazanov B, et al. Dppa2/4 facilitate epigenetic remodeling during reprogramming to pluripotency[J]. Cell Stem Cell, 2018, 23(3): 396-411.e8.
[32]	Pastor WA, Liu W, Chen D, et al. TFAP2C regulates transcription in human naive pluripotency by opening enhancers[J]. Nat Cell Biol, 2018, 20(5): 553-564.
[33]	Gorecka J, Kostiuk V, Fereydooni A, et al. The potential and limitations of induced pluripotent stem cells to achieve wound healing[J]. Stem Cell Res Ther, 2019, 10(1): 87.
[34]	Judson RL, Babiarz JE, Venere M, et al. Embryonic stem cell-specific microRNAs promote induced pluripotency[J]. Nat Biotechnol, 2009, 27(5): 459-461.
[35]	Oshima H, Rochat A, Kedzia C, et al. Morphogenesis and renewal of hair follicles from adult multipotent stem cells[J]. Cell, 2001, 104(2): 233-245.
[36]	Zhang J, Guan J, Niu X, et al. Exosomes released from human induced pluripotent stem cells-derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis[J]. J Transl Med, 2015, 13: 49.
[37]	Dash BC, Korutla L, Vallabhajosyula P, et al. Unlocking the potential of induced pluripotent stem cells for wound healing: the next frontier of regenerative medicine[J]. Adv Wound Care (New Rochelle), 2022, 11(11): 622-638.
[38]	Doeser MC, Scholer HR, Wu G. Reduction of fibrosis and scar formation by partial reprogramming in vivo[J]. Stem Cells, 2018, 36(8): 1216-1225.
[39]	Clayton ZE, Tan RP, Miravet MM, et al. Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model[J]. Biosci Rep, 2018, 38(4)：BSR20180563.
[40]	Wu R, Du D, Bo Y, et al. Hsp90alpha promotes the migration of iPSCs-derived keratinocyte to accelerate deep second-degree burn wound healing in mice[J]. Biochem Biophys Res Commun, 2019, 520(1): 145-151.
[41]	Yan Y, Jiang J, Zhang M, et al. Effect of iPSCs-derived keratinocytes on healing of full-thickness skin wounds in mice[J]. Exp Cell Res, 2019, 385(1): 111627.
[42]	Gill D, Parry A, Santos F, et al. Multi-omic rejuvenation of human cells by maturation phase transient reprogramming[J]. Elife, 2022, 11: e71624.
[43]	Sacco AM, Belviso I, Romano V, et al. Diversity of dermal fibroblasts as major determinant of variability in cell reprogramming[J]. J Cell Mol Med, 2019, 23(6): 4256-4268.
[44]	Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors[J]. Science, 2008, 322(5903): 949-953.
[45]	Romanazzo S, Lin K, Srivastava P, et al. Targeting cell plasticity for regeneration: From in vitro to in vivo reprogramming[J]. Adv Drug Deliv Rev, 2020, 161-162: 124-144.

Please choose a citation manager

Content to export