探究丝素蛋白（SF）修饰的羊脱细胞真皮基质（SADM）的理化性质及其与人脂肪间充质干细胞（hADSCs）膜片复合体系对小鼠全层皮肤缺损创面的修复效果及机制。

采用胰酶-核酶消化联合反复冻融制备微孔化SADM，以5.0%SF溶液浸渍修饰获得SF-SADM，扫描电子显微镜下观察修饰前后SADM孔隙结构的变化并测定孔隙率，采用体外降解实验观察修饰前后SADM的降解情况。从整形手术废弃脂肪中分离培养hADSCs，鉴定后进行膜片化培养，并分别与SADM和SF-SADM复合培养得到SADM-hADSCs和SF-SADM-hADSCs复合体系。采用MTS法对比SF修饰前后细胞增殖活性，荧光标记观察细胞膜片在支架上的黏附情况，激光共聚焦显微镜下观察细胞在支架上的迁移能力。取36只健康昆明小鼠，分为SADM组、SADM-hADSCs组及SF-SADM-hADSCs组，每组12只。于背部正中建立直径约1 cm圆形全层皮肤缺损模型，各组创面覆盖相应膜片。于术后第3、7、14 天观察创面愈合情况、组织学变化及血管生成情况。对数据行单因素方差分析及t检验。

经SF修饰后SADM孔隙率明显改善（P<0.05），SF在一定程度上延缓了酶解过程，使降解更可控。SF-SADM-hADSCs细胞增殖活性明显高于SADM-hADSCs（P<0.05）。体内促愈合实验中，SF-SADM-hADSCs组术后第14 天创面愈合率达96.3%±2.1%，显著高于SADM组的72.4%±4.2%和SADM-hADSCs组的85.7%±3.5%（P值均<0.05），基本实现再上皮化且血管化良好。

5.0%SF溶液可优化SADM的理化性质，使其更具生物活性。SF-SADM-hADSCs复合体系可通过促进再上皮化及血管生成，加速小鼠全层皮肤缺损创面高质量愈合。

To investigate the physicochemical properties of silk fibroin （SF）-modified sheep acellular dermal matrix （SADM），and the repair effect and mechanism of its composite system with human adipose-derived mesenchymal stem cell （hADSCs） membranes on full-thickness skin defect wounds in mice.

Microporous SADM was prepared by combining pancrease-ribozyme digestion with repeated freeze-thaw. SF-SADM was obtained by impregnation with 5.0% SF solution. Changes in SADM pore structure before and after modification were observed under scanning electron microscopy and porosity was measured. The degradation profiles of SADM before and after modification were evaluated via in vitro degradation experiments. The hADSCs were isolated and cultured from discarded adipose tissue obtained from plastic surgery. Following characterization，the hADSCs were subjected to sheet culture and subsequently co-cultured with SADM and SF-SADM to establish the SADM-hADSCs and SF-SADM-hADSCs composite system. MTS assay were used to compare cell proliferation activity before and after SF modification，fluorescence labeling was used to observe the adhesion of cell membranes to scaffolds，and laser confocal microscopy was used to observe cell migration ability on scaffolds. Thirty-six healthy Kunming mice were selected and approximately 1 cm diameter circular full-thickness skin defect model was established at the mid-dorsal area. The mice were divided into SADM，SADM-hADSCs，and SF-SADM-hADSCs groups，with 12 mice in each group. The wounds in each group were covered with the corresponding membrane. Wound healing，histological changes，and angiogenesis were observed at 3，7，and 14 days postoperatively，and one-way ANOVA and t-test were performed on the data.

After SF modification，SADM porosity was significantly improved （P<0.05）. SF delayed the enzymatic hydrolysis process to some extent，making the degradation more controllable. The cell proliferation activity of SF-SADM-hADSCs was significantly higher than that of SADM-hADSCs （P<0.05）. In vivo healing promoter experiments，the wound healing rate at 14 days after surgery in the SF-SADM-hADSCs group reached 96.3%±2.1%，significantly higher than 72.4%±4.2% in the SADM group and 85.7%±3.5% in the SADM-hADSCs group （P values all <0.05）, with essentially achieved re-epithelialization and good vascularization.

5.0% SF solution can optimize the physicochemical properties of SADM，making it more bioactive. The SF-SADM-hADSCs composite system can accelerate high-quality healing of full-thickness skin defect wounds in mice by promoting re-epithelialization and angiogenesis.