Inhibition of IGF-Ⅰ and IGF-ⅠR expression by ribozyme in inhibiting pathological scar formation

doi:10.3877/cma.j.issn.1673-9450.2017.01.006

Abstract

Abstract:

Objective

To investigate the function of insulin-like growth factor-Ⅰ(IGF-Ⅰ) and insulin-like growth factor-Ⅰ receptor(IGF-ⅠR) ribozyme in inhibiting pathological scar formation.

Methods

To select 100 SPF grade 4 to 6 week-old nude mice, and to select another 18 cases of orthopedics in the department of burns and plastics, Guangzhou Red Cross hospital affiliated to Jinan university from May, 2015 to July, 2016 for histopathological scar specimens. The fibroblasts were cultured in vitro and divided into 7 groups. After transfection of IGF-Ⅰ and IGF-ⅠR ribozymes, the hydroxyproline content in the culture supernatant was detected. The mRNA levels of IGF-Ⅰ and IGF-ⅠR were detected by real-time PCR. The mice were divided into A to H groups (n=10 each) randomly. The IGF-Ⅰ and IGF-ⅠR ribozymes were injected at different concentrations. The size of hypertrophic scar was detected by competitive PCR and the changes of type Ⅰ and Ⅲ procollagen mRNA levels in scar tissue. The levels of type Ⅰ and Ⅲ collagen were analysed by Western immunoblotting.

Results

The expression of IGF-Ⅰ and IGF-ⅠR mRNA in supernatant were decreased after transfection of IGF-Ⅰ and IGF-ⅠR ribozymes in fibroblasts. The expression of IGF-ⅠR mRNA was not significantly affected by transfection of IGF-ⅠR ribozyme alone or IGF-Ⅰ ribozyme alone. The expression of procollagen mRNA and type Ⅰ procollagen mRNA in the hypertrophic scars of nude mice was significantly lower than that of the control group (P<0.05). The expression of procollagen mRNA Levels and protein levels were increased.

Conclusion

IGF-Ⅰ and IGF-ⅠR ribozyme can be used for the treatment of pathological scar formation.

Key words: Insulin-like growth factor Ⅰ, Receptor, IGF type 1, Cicatrix, Ribozyme

Zhongyuan Deng, Xiaojian Li, Haijing Hou, Tao Zhang. Inhibition of IGF-Ⅰ and IGF-ⅠR expression by ribozyme in inhibiting pathological scar formation[J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2017, 12(01): 34-38.

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Figures/Tables 5

References 12

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组别	实验前	实验后
A组	28.74±1.85	25.93±2.15^ab
B组	30.78±2.51	27.58±2.66^ab
C组	28.74±1.06	26.77±1.97^ab
D组	30.25±3.61	29.28±2.87
E组	29.33±2.77	25.04±2.41^ab
F组	29.17±2.68	23.79±1.32^ab
G组	31.45±2.82	30.57±2.65
H组	32.58±1.37	32.98±1.99

组别	实验前	实验后
A组	28.74±1.85	25.93±2.15^ab
B组	30.78±2.51	27.58±2.66^ab
C组	28.74±1.06	26.77±1.97^ab
D组	30.25±3.61	29.28±2.87
E组	29.33±2.77	25.04±2.41^ab
F组	29.17±2.68	23.79±1.32^ab
G组	31.45±2.82	30.57±2.65
H组	32.58±1.37	32.98±1.99

组别	蛋白水平		mRNA水平
组别	Ⅰ型胶原	Ⅲ型胶原	Ⅰ型胶原	Ⅲ型胶原
A组	57.23±0.37^a	24.81±0.55^a	0.995±0.041^a	1.571±0.047^a
B组	59.56±0.24^a	25.42±0.49^a	0.942±0.032^a	1.485±0.059^a
C组	61.01±0.27^a	28.57±0.33^a	0.971±0.038^a	1.442±0.073^a
D组	63.34±0.61^a	29.53±0.25^a	0.947±0.021^a	1.647±0.094^a
E组	49.74±0.34^a	41.55±0.26^a	0.752±0.053^a	2.501±0.023^a
F组	45.76±0.17^a	41.39±0.31^a	0.772±0.048^a	2.615±0.095^a
G组	72.87±0.39	11.25±0.15	1.175±0.027	1.187±0.068
H组	82.75±0.36	19.31±0.22	1.259±0.064	1.377±0.081

组别	蛋白水平		mRNA水平
组别	Ⅰ型胶原	Ⅲ型胶原	Ⅰ型胶原	Ⅲ型胶原
A组	57.23±0.37^a	24.81±0.55^a	0.995±0.041^a	1.571±0.047^a
B组	59.56±0.24^a	25.42±0.49^a	0.942±0.032^a	1.485±0.059^a
C组	61.01±0.27^a	28.57±0.33^a	0.971±0.038^a	1.442±0.073^a
D组	63.34±0.61^a	29.53±0.25^a	0.947±0.021^a	1.647±0.094^a
E组	49.74±0.34^a	41.55±0.26^a	0.752±0.053^a	2.501±0.023^a
F组	45.76±0.17^a	41.39±0.31^a	0.772±0.048^a	2.615±0.095^a
G组	72.87±0.39	11.25±0.15	1.175±0.027	1.187±0.068
H组	82.75±0.36	19.31±0.22	1.259±0.064	1.377±0.081

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